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| Status |
Public on Aug 20, 2023 |
| Title |
Reconstruction of Dynamic Mammary Mini Gland in vitro for Normal Physiology and Oncogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Organoid culture has been extensively exploited for normal tissue reconstruction and disease modeling, however, it is still challenging to establish physiologically relevant architecture, size and functions in homeostasis. Here, we describe the development of a long-term adult stem cell derived mammary mini-gland culture system that supports robust 3D outgrowths recapitulating the morphology, cellular context, transcriptional heterogeneity of the normal mammary gland. The self-organization ability of stem cells and the stability of the outgrowths were determined by a coordinated combination of extracellular matrix, environmental signalings and dynamic physiological cycles. These mini glands are hormone responsive and reproduce the entire postnatal mammary development including puberty, estrous cycle, lactation and involution. Most intriguingly, these mini glands maintained the mammary stem cells and represented the fate transition from embryonic bipotency to postnatal unipotency in lineage tracing assays. In addition, induced oncogene expression in the mini glands drove the clinically relevant cancer initiation that can be monitored chronologically. Together, this study provides an experimental system fostering a dynamic organ miniature that can be studied chronologically and spatially for physiologically relevant biological processes with complexity.
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| Overall design |
Single cell RNA profiles of mammary gland basal cells and luminal cells from primary mammary gland and mammary mini gland
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/37919421/
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| Contributor(s) |
Yuan L, Xie S, Bai H, Liu X, Cai P, Lu J, Wang C, Lin Z, Li S, Guo Y, Cai S |
| Citation(s) |
37919421 |
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| Submission date |
Aug 18, 2022 |
| Last update date |
Nov 19, 2023 |
| Contact name |
Shang Cai |
| E-mail(s) |
caishang@westlake.edu.cn
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| Organization name |
Westlake University
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| Department |
School of Life Science
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| Lab |
Stem Cell and Cancer Laboratory
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| Street address |
Shilongshan Road No.18, Cloud Town, Xihu District
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| City |
HangZhou |
| State/province |
ZheJiang |
| ZIP/Postal code |
310013 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA870729 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211527_Barcode.txt.gz |
662 b |
(ftp)(http) |
TXT |
| GSE211527_RAW.tar |
2.9 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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