GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE21149 Query DataSets for GSE21149
Status Public on Jun 28, 2010
Title Mycobacterium bovis-BCG vaccination induces a specific pulmonary transcriptome biosignature in mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary In the present study, we applied microarray technology to define a biosignature from the whole genome expression in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before challenge, and biomarker patterns that correlated with anamnestic protective responses following exposure to virulent M. bovis. Further, Our aim was to define these markers to be detectable without in vitro antigenic challenge. After BCG vaccination, we defined a specific pulmonary gene expression signature related to the connective tissue development and function network that predicted vaccine success before M. bovis challenge. In addition, a Th17-related cytokine profile was found that correlated with vaccine-induced protective immunity following infection with virulent M. bovis in the lung as well as additional genes that were up-regulated in the spleens of vaccinated animals post-infection that was related to neutrophil biology and inflammation. This study has therefore prioritized both biomarkers predicting vaccination success before challenge and bio-signatures that are associated with protective immune responses that will be useful to evaluate future vaccine candidates.
Overall design Two groups of 20 mice each were immunised by a single intradermal injection of 2 x 10 to 5 CFU of M. bovis BCG (Vaccinated), or Hanks buffered salt solution (HBSS) (Unvaccinated). Six weeks later 5 mice from each group were euthanized for immunological analyses and the remaining mice from each group were challenged with approx 600 CFU M. bovis via the intranasal route. At days 3 and 14 post challenge five mice per group were euthanized and spleens and lungs harvested.
Contributor(s) Aranday-Cortes E, Kaveh D, Nunez-Garcia J, Hogarth PJ, Vordermeier M
Citation(s) 20596522
Submission date Mar 31, 2010
Last update date Jan 12, 2017
Contact name ELIHU Aranday-Cortes
Organization name Central Veterinary Laboratories Agency-Weybridge
Department SEB 4
Lab TB Research Group
Street address Woodham Lane, New Haw
State/province SURREY
ZIP/Postal code KT153NB
Country United Kingdom
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (57)
GSM529495 ExvivoLung_UU_1
GSM529496 ExvivoLung_UU_2
GSM529497 ExvivoLung_UU_4
BioProject PRJNA126627

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21149_RAW.tar 460.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap