NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE210558 Query DataSets for GSE210558
Status Public on Feb 03, 2023
Title Nab6 and Mrn1 regulate the expression of cell wall mRNAs in budding yeast
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Other
Summary The fungal cell wall provides protection and structure to cells, and serves as an important target for antifungal compounds. The cell wall integrity (CWI) signaling pathway regulates the transcriptional response to various cell wall stresses, but emerging evidence suggests posttranscriptional pathways play an important complementary role. Here, we show that the RNA-binding proteins (RBPs) Mrn1 and Nab6 specifically target the 3′ UTRs of a largely overlapping set of cell wall-associated mRNAs. These mRNAs are downregulated in the absence of Nab6, indicating that Nab6 directly or indirectly stabilizes its target mRNAs. We further show that Nab6 acts in parallel to conventional CWI signaling to maintain adequate expression of cell wall mRNAs. Cells lacking both pathways are hypersensitive to antifungal compounds targeting the cell wall, while deletion of MRN1 partially alleviates the growth and molecular phenotypes associated with the Dnab6 strain. Taken together, our results uncover a novel posttranscriptional pathway which mediates resistance to cell wall stress and antifungal compounds.
 
Overall design There are 4 CRAC datasets and 50 RNAseq datasets. The CRAC datasets were generated using affinity tagged versions of Mrn1 and Nab6 under standard ('control') conditions in otherwise wild type cells. The RNAseq experiments were prepared from wild type, Dmrn1, Dnab6, Dslt2, and Dnab6Dslt2 cells before and after treatment with 2 µg/mL Congo Red (0, 1, 2, and 4hr). In addition, wild type, Dmrn1, Dnab6, and Dmrn1Dnab6 cells were treated with 60µg/mL Congo Red (0 and 2 hr).
 
Contributor(s) Bresson S, Shchepachev V, Tollervey D
Citation(s) 36862555
Submission date Aug 04, 2022
Last update date May 05, 2023
Contact name Stefan Bresson
E-mail(s) stefan.bresson@gmail.com
Organization name University of Edinburgh
Department Wellcome Trust Centre for Cell Biology
Lab Tollervey Lab
Street address Max Born Crescent, Swann 5.1
City Edinburgh
State/province Scotland
ZIP/Postal code EH9 3BF
Country United Kingdom
 
Platforms (1)
GPL19756 Illumina NextSeq 500 (Saccharomyces cerevisiae)
Samples (54)
GSM6432468 01_wt_0min
GSM6432469 02_wt_2CR_60min
GSM6432470 03_wt_2CR_120min
Relations
BioProject PRJNA866160

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE210558_RAW.tar 1.9 Gb (http)(custom) TAR (of BEDGRAPH, BW, TXT)
GSE210558_Saccharomyces_cerevisiae_EF4.74_introns_removed.fasta.gz 3.3 Mb (ftp)(http) FASTA
GSE210558_intronless_genome_annotation.gtf.gz 245.8 Kb (ftp)(http) GTF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap