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Series GSE210553 Query DataSets for GSE210553
Status Public on Feb 17, 2023
Title Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells [bulk RNA-seq]
Organism Callithrix jacchus
Experiment type Expression profiling by high throughput sequencing
Summary Reconstitution of germ cell fate from pluripotent stem cells provide the opportunities to understand the molecular underpinning of germ cell development. Here, we established a several culture methods of induced pluripotent stem cells (iPSCs) in common marmoset (Callithrix jacchus, cj), which stably propagate in an undifferentiated state. Notably, iPSCs cultured on feeder layer in the presence of a WNT signaling inhibitor upregulate genes related to ubiquitin-dependent protein catabolic process and acquire permissive state that readily differentiate into primordial germ cell-like cells (PGCLCs) bearing immunophenotypic and transcriptomic similarities to pre-migratory cjPGCs. Induction of cjPGCLCs accompanies transient upregulation of mesodermal genes and culminate in establishing primate-specific transcriptional network similar to humans and cynomolgus monkeys. Moreover, cjPGCLCs can be expanded in monolayer while retaining cellular state. Upon co-culture with mouse testicular somatic cells, these cells acquire DDX4+ early prospermatogonia-like state. Our findings provide a framework for understanding and reconstituting germ cell development in marmoset in vitro, which serve as an comparative tool and foundation for a preclinical model for human in vitro gametogenesis.
 
Overall design CjiPSCs cultured in the presence or absence of feeders, in the presence or absence of IWR1, were collected. Extension culture of cjPGCLCs were collected at day 30.
 
Contributor(s) Cheng K
Citation(s) 36719274
Submission date Aug 04, 2022
Last update date Jun 02, 2023
Contact name keren cheng
E-mail(s) keren.cheng@outlook.com
Organization name Zhejiang University
Department School of Medicine
Street address 1575 chouzhou bei road
City Yiwu
State/province Zhejiang
ZIP/Postal code 320000
Country China
 
Platforms (1)
GPL17712 Illumina HiSeq 2000 (Callithrix jacchus)
Samples (16)
GSM6432357 FF cjiPSCs, rep1
GSM6432358 FF cjiPSCs, rep2
GSM6432359 FF/IWR1 cjiPSCs, rep1
This SubSeries is part of SuperSeries:
GSE210576 Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells
Relations
BioProject PRJNA866149

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE210553_RPFK_all.sample.csv.gz 2.4 Mb (ftp)(http) CSV
GSE210553_raw.counts_all.sample.csv.gz 716.0 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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