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| Status |
Public on Aug 05, 2022 |
| Title |
Target genes of transcription factor Myc regulated by Mycobacterium tuberculosis EST12 |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Target genes of transcription factor Myc regulated by Mycobacterium tuberculosis EST12
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| Overall design |
RAW264.7 cells (1×107) were stimulated with 2 μM EST12 for 1 h, then treated or untreated RAW264.7 cells were collected and sent to DIATRE Biotechnology (Shanghai, China) in dry ice for CUT&Tag sequencing. Briefly, in this method, a highly active Tn5 transposase is fused to Protein A, and a library-building linker primer is loaded to form a pA-Tn5 transposition complex. Under the guidance of Myc antibody (10828-1-AP, Proteintech, China), the pA-Tn5 transposition complex can target and cut the DNA sequence near Myc-bound region.
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| Contributor(s) |
Wu J, Zhang X |
| Citation(s) |
36003390 |
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| Submission date |
Jul 29, 2022 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Jian Wu |
| E-mail(s) |
jianwu@whu.edu.cn
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| Organization name |
wuhan university
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| Street address |
donghu road
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| City |
wuhan |
| ZIP/Postal code |
430071 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA863633 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE210088_RAW.tar |
64.4 Mb |
(http)(custom) |
TAR (of BW, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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