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| Status |
Public on Jul 29, 2022 |
| Title |
Influence of Sox4 on chromatin accessibility of adult hepatocytes |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Recent studies have demonstrated that hepatocytes can be reprogrammed into biliary epithelial cell-like cells. An earlier work in our laboratory nominated Sox4 as an initiation factor of this reprogramming event To investigate the effect of Sox4 on the change of chromatin landscape of hepatocytes, we performed ATAC-seq of Sox4-overexpressed (OE) hepatocytes.
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| Overall design |
Mice were injected with adeno-associated viruses (AAVs) packaged with mouse Sox4 (AAV8-TBG-HA-Sox4-P2A-Cre) or empty virus (AAV8-TBG-Cre), and hepatocytes were harvested 4 days post injection by a standard 2-step liberase digestion method followed by a Percoll enrichment step.
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| Contributor(s) |
Katsuda T, Sussman J, Stanger BZ |
| Citation(s) |
36037289 |
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| Submission date |
Jul 22, 2022 |
| Last update date |
Nov 30, 2022 |
| Contact name |
Takeshi Katsuda |
| E-mail(s) |
tkatsuda412@gmail.com
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| Phone |
+1-215-746-5559
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| Organization name |
University of Pennsylvania
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| Department |
Perelman School of Medicine
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| Lab |
Gastroenterology Division
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| Street address |
421 Curie Blvd, BRB II/III Rm531
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA861256 |
