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Series GSE20913 Query DataSets for GSE20913
Status Public on Mar 17, 2010
Title CD44+ or CD44– tumor cells isolated from K19-Wnt1/C2mE mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary We hypothesized that CD44+ tumor cells might have an increased capacity for reactive oxygen species (ROS) defense in the gastric tumors of K19-Wnt1/C2mE mice. To address this possibility, we examined the expression of antioxidant genes in tumor cells isolated from K19-Wnt1/C2mE mice by fluorescence-activated cell sorting (FACS). Lineage marker (Lin)–negative cells that were CD44+ or CD44– were thus isolated from the gastric tumors of 30-week-old K19-Wnt1/C2mE mice and subjected to cDNA microarray analysis. The expression of several key antioxidant genes, including those for glutathione peroxidase (GPX) and peroxiredoxin (PRDX) isoforms, was found to be increased in CD44+ tumor cells compared with that in CD44– cells.
 
Overall design Lineage marker (Lin)–negative cells that were CD44+ or CD44– were isolated from the gastric tumors of 30-week-old K19-Wnt1/C2mE mice. Total RNA was isolated and subjected to cDNA microarray analysis. Two CD44+ replicates and two CD44- replicates were examined.
 
Contributor(s) Ishimoto T, Nagano O, Saya H
Citation(s) 21397861, 23848514
Submission date Mar 16, 2010
Last update date Nov 24, 2020
Contact name Osamu Nagano
E-mail(s) osmna@sb3.so-net.ne.jp
Phone +81-3-5363-3983
Fax +81-3-5363-3982
Organization name Keio University School of Medicine
Department Institute for Advanced Medical Research
Lab Division of Gene Regulation
Street address 35 Shinanomachi
City Tokyo
State/province DDS PhD
ZIP/Postal code 160-8582
Country Japan
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (4)
GSM523201 CD44(+), 1
GSM523202 CD44(+), 2
GSM523203 CD44(-), 1
Relations
BioProject PRJNA124455

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20913_RAW.tar 7.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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