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| Status |
Public on Jul 22, 2022 |
| Title |
Self-reactivity of CD8 T-cell clones determines their differentiation status rather than their responsiveness in infections |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The peripheral blood mononuclear cells (PBMCs) from young and old C57BL/6J mice were single cell RNA sequenced along with V(D)J sequences and cell surface protein markers that were used to demultiplex them from separate and unrelated experiment. The resulting data were then used to create an atlas of mouse peripheral CD8+ T cells. The analysis of this atlas has revealed two unconventional populations of antigen-inexperienced T cells and allowed for further examination of the steady-state phenotype of monoclonal T cells with various levels of self-reactivity.
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| Overall design |
Peripheral CD8+ T cells from young (n = 3, age = 8 weeks) and old C57BL/6J(n = 3, age = 88 weeks) WT mice were sorted (Influx), scRNA sequenced and analyzed by for gene expression (two technical replicates), V(D)J and cell surface markers (used for demultiplexing of sequences from unrelated experiment).
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| Contributor(s) |
Paprckova D, Niederlova V, Neuwirth A, Michalik J, Stepanek O |
| Citation(s) |
36275704 |
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| Submission date |
Jul 21, 2022 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Juraj Michalik |
| E-mail(s) |
juraj.michalik@img.cas.cz
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| Organization name |
Institute of Molecular Genetics of the Czech Academy of Sciences
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| Lab |
Lab of Adaptive Immunity
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| Street address |
Vídeňská 1083
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| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA861004 |
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