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Series GSE20758 Query DataSets for GSE20758
Status Public on Apr 10, 2010
Title Expression data from LCM captured prostate cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The prostate represents a complex mix of cell types and there is a need to analyze distinct cell populations to better understand their potential interactions. This study of cell-type specific gene expression patterns will contribute to understanding of how tumor epithelial cells may be affected by adjacent interstitial stromal cells within the tumor microenvirnonment.
LCM and microarray analysis were used to identify gene expression patterns in prostate cancer epithelial and interstitial stromal cell populations. More than 500 genes whose expression levels were significantly different in epithelial cells versus adjacent stromal tissue were identified. Several chemokines and growth factors were more highly expressed in prostate cancer stromal cells while several transcription factors and receptors were more highly expressed in epithelial cells.
Overall design Five micron serial sections from three different intermediate grade prostate tumors were provided. One section from each had been stained by hematoxylin and eosin and the tumor areas marked for identification. To obtain RNA from 2 different tissue types (glandular epithelium and interstitial stroma) tumor areas observed to have abundant cells of interest were identified. Tumor epithelium and adjacent stromal tissue were collected separately from each slide. We sought to obtain similar populations of cells of each tissue type, so to that end, we examined each slide and and marked areas to direct the laser capture. Approximately 1,000 cells were captured per cap and RNA was extracted and hybridized to Affymetrix microarrays as recommended by manufacturer.
Contributor(s) Fraizer GC, Mintz EM, Piontkivska H, Gregg JL, Brown KE
Citation(s) 20426842
Submission date Mar 11, 2010
Last update date Dec 06, 2018
Contact name Gail C Fraizer
Phone 330-671-1398
Fax 330-672-3713
Organization name Kent State University
Department Biological Sciences
Street address 251 Cunningham Hall
City kent
State/province OH
ZIP/Postal code 44242
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (6)
GSM520769 epithelial cells (PC-A5)
GSM520770 interstitial stromal cells (PC-D9)
GSM520771 epithelial cells (PC-A6)
BioProject PRJNA125047

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20758_RAW.tar 11.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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