|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Nov 10, 2023 |
Title |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
How CD4+ lineage gene expression is initiated in differentiating thymocytes remains poorly understood. Here, we show that the paralog transcription factors Zfp281 and Zfp148 control both this process and cytokine expression by T helper cell type 2 (TH2) effector cells. Genetic, single-cell, and spatial transcriptomic analyses showed that these factors promote the intrathymic CD4+ T cell differentiation of class II major histocompatibility complex (MHC II)–restricted thymocytes, including expression of the CD4+ lineage–committing factor Thpok. In peripheral T cells, Zfp281 and Zfp148 promoted chromatin opening at and expression of TH2 cytokine genes but not of the TH2 lineage–determining transcription factor Gata3. We found that Zfp281 interacts with Gata3 and is recruited to Gata3 genomic binding sites at loci encoding Thpok and TH2 cytokines. Thus, Zfp148 and Zfp281 collaborate with Gata3 to promote CD4+ T cell development and TH2 cell responses.
This SuperSeries is composed of the SubSeries listed below.
|
|
|
Overall design |
Refer to individual Series
|
Web link |
https://www.science.org/doi/10.1126/sciimmunol.adi9066
|
|
|
Citation(s) |
37948511 |
|
Submission date |
Jul 06, 2022 |
Last update date |
Nov 13, 2023 |
Contact name |
Remy Bosselut |
E-mail(s) |
bosselur@helix.nih.gov
|
Phone |
240-760-6866
|
Organization name |
National Cancer Institute, National Institute of Health
|
Department |
Laboratory of Immune Cell Biology
|
Lab |
Bosselut
|
Street address |
9000 Rockville Pike, Building 37
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platforms (3) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
GPL30172 |
NextSeq 2000 (Mus musculus) |
|
Samples (38)
|
GSM6255773 |
dKO, thymocyte, rep2 |
GSM6256699 |
LCZ1 Ctrl ImCD4 Thymocyte |
GSM6256700 |
LCZ2 Ctrl ImCD4 Thymocyte |
GSM6256701 |
LCZ3 Ctrl ImCD4 Thymocyte |
GSM6256702 |
LCZ4 Zfp148/281 dKO ImCD4 thymocyte |
GSM6256703 |
LCZ5 Zfp148/281 dKO ImCD4 thymocyte |
GSM6256704 |
LCZ6 Zfp148/281 dKO ImCD4 thymocyte |
GSM6256705 |
LCZ7 Ctrl ImCD8 thymocyte |
GSM6256706 |
LCZ8 Ctrl ImCD8 thymocyte |
GSM6256707 |
LCZ9 Ctrl ImCD8 thymocyte |
GSM6256708 |
LCZ10 Zfp148/281 dKO ImCD8 thymocyte |
GSM6256709 |
LCZ11 Zfp148/281 dKO ImCD8 thymocyte |
GSM6256710 |
LCZ12 Zfp148/281 dKO ImCD8 thymocyte |
GSM6288249 |
ChIP DNA, 2% Input rep 1 |
GSM6288250 |
ChIP DNA, IgG Control |
GSM6288251 |
ChIP DNA, aZfp281 rep1 |
GSM7093285 |
Th2_WT_Gata3 |
GSM7093286 |
Th2_tdKO_Gata3 |
GSM7093287 |
Th2_tdKO_Zfp281_cpm.bw |
GSM7097899 |
Ctrl, dLN, rep1_atac |
GSM7097900 |
Ctrl, dLN, rep1_rna |
GSM7097901 |
pdKO, dLN, rep1_atac |
GSM7097902 |
pdKO, dLN, rep1_rna |
GSM7097903 |
Ctrl, dLN, rep2_atac |
GSM7097904 |
Ctrl, dLN, rep2_rna |
GSM7097905 |
pdKO, dLN, rep2_atac |
GSM7097906 |
pdKO, dLN, rep2_rna |
GSM7097913 |
Ctrl, bio1, tech1 |
GSM7097914 |
tdKO, bio1, tech1 |
GSM7097915 |
Ctrl, bio1, tech2 |
GSM7097916 |
tdKO, bio1, tech2 |
GSM7097917 |
Ctrl, bio2 |
GSM7097918 |
tdKO, bio2 |
GSM7655561 |
Th2_ChIP_IgG_Rep2 |
GSM7655562 |
Th2_ChIP_aZfp281_Rep2 |
|
This SuperSeries is composed of the following SubSeries:
|
GSE206521 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [scRNA-seq] |
GSE206542 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Population RNA-seq] |
GSE207469 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions. (ChIP-seq) |
GSE227330 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Papain multiome] |
GSE227332 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Thymus Visium] |
GSE237865 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Th2 ChIP] |
|
Relations |
BioProject |
PRJNA856244 |
Supplementary file |
Size |
Download |
File type/resource |
GSE207572_RAW.tar |
7.8 Gb |
(http)(custom) |
TAR (of BW, CSV, H5, JPG, JSON, PNG, TAB, TBI, TIFF, TSV) |
SRA Run Selector |
|
|
|
|
|