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| Status |
Public on Mar 10, 2010 |
| Title |
mRNA and microRNA microarray analysis on human Jurkat T cells exposed to silver nanoparticles and silver ions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
To identify genes and pathways involved in AgNPs and Ag ion toxicity, mRNA microarray analysis was conducted on human Jurkat T cells. The results indicate that more DEGs were induced by AgNPs than by Ag ion and AgNPs induced gene expression were not clustered with control and Ag ion induced ones. DEG analysis indicated that metallothionein (MT) 2A, 1H, 1F, and 1A and endonucleases G like 1 (ENDOGL1) were upregulated by AgNPs exposure more than 2 folds compared to control.
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| Overall design |
Jurkat T cells were exposed to 0.2 mg/L of AgNPs and Ag ions for 24 h. After treatment, total RNA was extracted and mRNA microarray was conducted on control, AgNPs treated and Ag ion treated Jurktat T cells. Microarray analysis were performed in triplicate.
Jurkat T cells were exposed to 0.2 mg/L of AgNPs and Ag ions for 24 h. After treatment, total RNA was extracted and miRNA microarray was conducted on control, AgNPs treated and Ag ion treated Jurktat T cells.
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| Contributor(s) |
Choi J |
| Citation(s) |
20932003, 24974767 |
| Submission date |
Mar 09, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
jinhee choi |
| E-mail(s) |
jinhchoi@uos.ac.kr
|
| Phone |
82-2-2210-5622
|
| Organization name |
University of Seoul
|
| Department |
College of Urban Science
|
| Lab |
Faculty of Environmental Engineering
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| Street address |
90 Jeonnong-dong, Dongdaemun-gu
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| City |
seoul |
| ZIP/Postal code |
130-743 |
| Country |
South Korea |
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| Platforms (2) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
| GPL8179 |
Illumina Human v2 MicroRNA expression beadchip |
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| Samples (12)
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| GSM519531 |
mRNA Jurkat 24hr AgNPs exposure rep1 |
| GSM519532 |
mRNA Jurkat 24hr AgNPs exposure rep2 |
| GSM519533 |
mRNA Jurkat 24hr AgNPs exposure rep3 |
| GSM519534 |
mRNA Jurkat 24hr Ag ions exposure rep1 |
| GSM519535 |
mRNA Jurkat 24hr Ag ions exposure rep2 |
| GSM519536 |
mRNA Jurkat 24hr Ag ions exposure rep3 |
| GSM519537 |
microRNA Jurkat not exposed rep1 |
| GSM519538 |
microRNA Jurkat 24hr AgNPs exposure rep1 |
| GSM519539 |
microRNA Jurkat 24hr Ag ions exposure rep1 |
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| Relations |
| BioProject |
PRJNA125007 |
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