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Series GSE206466 Query DataSets for GSE206466
Status Public on Nov 29, 2022
Title DAZL regulates proliferation of human primordial germ cell by direct binding to precursor miRNAs and enhances DICER processing activity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Understanding the molecular and cellular mechanisms of human primordial germ cells (hPGCs) is essential in studying infertility and germ cell tumorigenesis. Many RNA binding proteins (RBPs) and non-coding RNAs are specifically expressed and functional during hPGC developments. However, the roles and regulatory mechanisms of these RBPs and non-coding RNAs, such as microRNAs (miRNAs), in hPGCs remain elusive. In this study, we reported a new regulatory function of DAZL, a germ cellspecific RBP, in miRNA biogenesis and cell proliferation. First, DAZL colocalized with miRNA let-7a in human PGCs and upregulated the levels of over a hundred mature miRNAs, including eight out of nine let-7 family, miR10, and miR199. Purified DAZL directly bound to the loops of precursor miRNAs with sequence specificity of GUU. The binding of DAZL to the precursor miRNA increased the maturation of miRNA by enhancing the cleavage activity of DICER. Furthermore, cell proliferation assay and cell cycle analysis confirmed that DAZL inhibited the proliferation of in vitro PGCs by promoting the maturation of these miRNAs. Evidently, the mature miRNAs upregulated by DAZL silenced cell proliferation regulators including TRIM71. Moreover, DAZL inhibited germline-tumor-cell proliferation and teratoma formation. These results demonstrate that DAZL regulates hPGC proliferation by enhancing miRNA processing.
Overall design Comparative gene expression profiling analysis of RNA-seq data for WT PGCLCs and oeDAZL PGCLCs and expression profiling analysis of miRNA-seq for WT PGCLCs and its derivatives (shDAZL_4,shDAZL_6).
Contributor(s) Xiaoyu F, Shuting X
Citation(s) 36273819
Submission date Jun 20, 2022
Last update date Nov 29, 2022
Contact name XIAOYU FENG
Phone +8618810058126
Organization name Tsinghua University
Street address QINGHUAYUAN street´╝îHAIDIAN distribute
State/province BEIJING
ZIP/Postal code 100084
Country China
Platforms (2)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (10)
GSM6254995 Normal_rep1
GSM6254996 Normal_rep2
GSM6254997 oeDAZL_rep1
BioProject PRJNA851041

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Supplementary file Size Download File type/resource
GSE206466_mirna_count.xlsx 171.0 Kb (ftp)(http) XLSX
GSE206466_pgclcs_expr_tpm.txt.gz 1.2 Mb (ftp)(http) TXT
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