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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 30, 2023 |
| Title |
Base editor induced mutation spectrum in GFP, human ATP1a1, human TP53BP1 and murine MyoD |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
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| Summary |
Analysis of targeted genomic and epigenomic mutations within GFP, human ATP1a1, human TP53BP1 and murine MyoD induced through state-of the art base editors
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| Overall design |
Genomic DNA was isolated from human HEK293T cells which constiutively express GFP or murine 3T3 cells which were transfected with the base editor and a gene-specific gRNA. Specific gene locus was amplified with barcoded primers and subjected to amplicon deep sequencing with either through PacBio Sequencing or Illumina Sequencing
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| Contributor(s) |
Weischedel JD, Higgins L, Rogers S, Chahwan R |
| Citation(s) |
37994786 |
| |
| Submission date |
Jun 20, 2022 |
| Last update date |
Aug 12, 2024 |
| Contact name |
Richard Chahwan |
| E-mail(s) |
richard.chahwan@uzh.ch
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| Organization name |
University of Zurich
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| Department |
Institute of Experimental Immunology
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| Lab |
Irchel Y23K74
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| Street address |
Winterthurerstrasse 190
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| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (3) |
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| Samples (134)
|
| GSM6254076 |
M1 + R2 + Booster_biol, rep1 |
| GSM6254077 |
C + R1_biol, rep1 |
| GSM6254078 |
C + R2_biol, rep1 |
| GSM6254079 |
C + R1 + Booster_biol, rep1 |
| GSM6254080 |
C + R2 + Booster_biol, rep1 |
| GSM6254081 |
Exp_PBS_GFP_Untreated_biol, rep1 |
| GSM6254082 |
M1 + R1_biol, rep2 |
| GSM6254083 |
M1 + R2_biol, rep2 |
| GSM6254084 |
M1 + R1 + Booster_biol, rep2 |
| GSM6254085 |
M1 + R2 + Booster_biol, rep2 |
| GSM6254086 |
C + R1_biol, rep2 |
| GSM6254087 |
C + R2_biol, rep2 |
| GSM6254088 |
C + R1 + Booster_biol, rep2 |
| GSM6254089 |
C + R2 + Booster_biol, rep2 |
| GSM6254090 |
Exp_PBS_GFP_Untreated_biol, rep2 |
| GSM6254091 |
M1 + R1_biol, rep3 |
| GSM6254092 |
M1 + R2_biol, rep3 |
| GSM6254093 |
M1 + R1 + Booster_biol, rep3 |
| GSM6254094 |
M1 + R2 + Booster_biol, rep3 |
| GSM6254095 |
C + R1_biol, rep3 |
| GSM6254096 |
C + R2_biol, rep3 |
| GSM6254097 |
C + R1 + Booster_biol, rep3 |
| GSM6254098 |
C + R2 + Booster_biol, rep3 |
| GSM6254099 |
Exp_PBS_GFP_Untreated_biol, rep3 |
| GSM6254100 |
M1 + gV3_biol, rep1 |
| GSM6254101 |
M2 + gV3_biol, rep1 |
| GSM6254102 |
M3 + gV3_biol, rep1 |
| GSM6254103 |
M4 + gV3_biol, rep1 |
| GSM6254104 |
C + gV3_biol, rep1 |
| GSM6254105 |
M1 + gV3_biol, rep2 |
| GSM6254106 |
M2 + gV3_biol, rep2 |
| GSM6254107 |
M3 + gV3_biol, rep2 |
| GSM6254108 |
M4 + gV3_biol, rep2 |
| GSM6254109 |
C + gV3_biol, rep2 |
| GSM6254110 |
M1 + gV3_biol, rep3 |
| GSM6254111 |
M2 + gV3_biol, rep3 |
| GSM6254112 |
M3 + gV3_biol, rep3 |
| GSM6254113 |
M4 + gV3_biol, rep3 |
| GSM6254114 |
C + gV3_biol, rep3 |
| GSM6254115 |
M1 + L1_biol, rep1 |
| GSM6254116 |
M1 + L2_biol, rep1 |
| GSM6254117 |
M1 + L1 + Booster_biol, rep1 |
| GSM6254118 |
M1 + L2 + Booster_biol, rep1 |
| GSM6254119 |
M1 + L1_biol, rep2 |
| GSM6254120 |
M1 + L2_biol, rep2 |
| GSM6254121 |
M1 + L1 + Booster_biol, rep2 |
| GSM6254122 |
M1 + L2 + Booster_biol, rep2 |
| GSM6254123 |
M1 + L1_biol, rep3 |
| GSM6254124 |
M1 + L2_biol, rep3 |
| GSM6254125 |
M1 + L1 + Booster_biol, rep3 |
| GSM6254126 |
M1 + L2 + Booster_biol, rep3 |
| GSM6254127 |
C + L1_biol, rep1 |
| GSM6254128 |
C + L2_biol, rep1 |
| GSM6254129 |
C + L1 + Booster_biol, rep1 |
| GSM6254130 |
C + L2 + Booster_biol, rep1 |
| GSM6254131 |
C + L1_biol, rep2 |
| GSM6254132 |
C + L2_biol, rep2 |
| GSM6254133 |
C + L1 + Booster_biol, rep2 |
| GSM6254134 |
C + L2 + Booster_biol, rep2 |
| GSM6254135 |
C + L1_biol, rep3 |
| GSM6254136 |
C + L2_biol, rep3 |
| GSM6254137 |
C + L1 + Booster_biol, rep3 |
| GSM6254138 |
C + L2 + Booster_biol, rep3 |
| GSM6254139 |
M1 + L1/2_biol, rep1 |
| GSM6254140 |
M2 + L1/2_biol, rep1 |
| GSM6254141 |
M3 + L1/2_biol, rep1 |
| GSM6254142 |
M4 + L1/2_biol, rep1 |
| GSM6254143 |
M1 + L1/2_biol, rep2 |
| GSM6254144 |
M2 + L1/2_biol, rep2 |
| GSM6254145 |
M3 + L1/2_biol, rep2 |
| GSM6254146 |
M4 + L1/2_biol, rep2 |
| GSM6254147 |
M1 + L1/2_biol, rep3 |
| GSM6254148 |
M2 + L1/2_biol, rep3 |
| GSM6254149 |
M3 + L1/2_biol, rep3 |
| GSM6254150 |
M4 + L1/2_biol, rep3 |
| GSM6254151 |
C + L1/2_biol, rep1 |
| GSM6254152 |
C + L1/2+ Booster_biol, rep1 |
| GSM6254153 |
C + L1/2_biol, rep2 |
| GSM6254154 |
C + L1/2+ Booster_biol, rep2 |
| GSM6254155 |
C + L1/2_biol, rep3 |
| GSM6254156 |
C + L1/2+ Booster_biol, rep3 |
| GSM6254157 |
M1 + L1/2 + Booster_biol, rep1 |
| GSM6254158 |
M2 + L1/2 + Booster_biol, rep1 |
| GSM6254159 |
M3 + L1/2 + Booster_biol, rep1 |
| GSM6254160 |
M4 + L1/2 + Booster_biol, rep1 |
| GSM6254161 |
M1 + L1/2 + Booster_biol, rep2 |
| GSM6254162 |
M2 + L1/2 + Booster_biol, rep2 |
| GSM6254163 |
M3 + L1/2 + Booster_biol, rep2 |
| GSM6254164 |
M4 + L1/2 + Booster_biol, rep2 |
| GSM6254165 |
M1 + L1/2 + Booster_biol, rep3 |
| GSM6254166 |
M2 + L1/2 + Booster_biol, rep3 |
| GSM6254167 |
M3 + L1/2 + Booster_biol, rep3 |
| GSM6254168 |
M4 + L1/2 + Booster_biol, rep3 |
| GSM6254169 |
Exp_ATP1A1_Untreated_biol, rep1 |
| GSM6254170 |
M1 + g1/A_biol, rep1 |
| GSM6254171 |
M1 + g1/A + Booster_biol, rep1 |
| GSM6254172 |
Exp_A_Untreated_biol, rep2 |
| GSM6254173 |
M1 + g1/A_biol, rep2 |
| GSM6254174 |
M1 + g1/A + Booster_biol, rep2 |
| GSM6254175 |
Exp_ATP1A1_Untreated_biol, rep3 |
| GSM6254176 |
M1 + g1/A_biol, rep3 |
| GSM6254177 |
M1 + g1/A + Booster_biol, rep3 |
| GSM6254178 |
Exp_53BP1_Untreated_biol, rep1 |
| GSM6254179 |
M1 + gB_biol, rep1 |
| GSM6254180 |
M1 + gB + Booster_biol, rep1 |
| GSM6254181 |
Exp_53BP1_Untreated_biol, rep2 |
| GSM6254182 |
M1 + gB_biol, rep2 |
| GSM6254183 |
M1 + gB + Booster_biol, rep2 |
| GSM6254184 |
Exp_53BP1_Untreated_biol, rep3 |
| GSM6254185 |
M1 + gB_biol, rep3 |
| GSM6254186 |
M1 + gB + Booster_biol, rep3 |
| GSM6254187 |
Exp_CH12_Untreated_biol, rep1 |
| GSM6254188 |
M1 + gV2/gV3/gV6/gV7_biol, rep1 |
| GSM6254189 |
M1 + gV2/gV3/gV6/gV7 + Booster_biol, rep1 |
| GSM6254190 |
C + gV2/gV3/gV6/gV7_biol, rep1 |
| GSM6254191 |
Exp_CH12_Untreated_biol, rep2 |
| GSM6254192 |
M1 + gV2/gV3/gV6/gV7_biol, rep2 |
| GSM6254193 |
M1 + gV2/gV3/gV6/gV7 + Booster_biol, rep2 |
| GSM6254194 |
C + gV2/gV3/gV6/gV7_biol, rep2 |
| GSM6254195 |
Exp_CH12_Untreated_biol, rep3 |
| GSM6254196 |
M1 + gV2/gV3/gV6/gV7_biol, rep3 |
| GSM6254197 |
C + gV2/gV3/gV6/gV7_biol, rep3 |
| GSM6254198 |
Exp_MyoD_Untreated |
| GSM6254199 |
Exp_MyoD_M1 + gM |
| GSM6254200 |
Exp_MyoD_M4 + gM |
| GSM6254201 |
Illumina_M1+gR1_biol, rep_1 |
| GSM6254202 |
Illumina_M1+gR1+Booster_biol, rep_1 |
| GSM6254203 |
Illumina_M1+gR1_biol, rep_2 |
| GSM6254204 |
Illumina_M1+gR1+Booster_biol, rep_2 |
| GSM6254205 |
Illumina_M1+gR1_biol, rep_3 |
| GSM6254206 |
Illumina_M1+gR1+Booster_biol, rep_3 |
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| Relations |
| BioProject |
PRJNA850939 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206445_RAW.tar |
1.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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