GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE20633 Query DataSets for GSE20633
Status Public on Mar 05, 2010
Title Exogenous expression of CC3/TIP30 in U373 cells with or without UV treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The pro-apoptotic protein CC3/TIP30 has an unusual cellular function as an inhibitor of nucleocytoplasmic transport. This function is likely to be activated under conditions of stress. Here we examined the potential role of CC3 in regulation of cellular responses to genotoxic stress. We found that forced expression of CC3 in CC3-negative cells delays repair of both UV-induced and oxidative DNA damage. Exogenously introduced CC3 negatively affects expression levels of DDB2 and p21CIP11 and inhibits induction of c-FOS after UV exposure, which is likely to contribute to the observed delay in DNA damage repair. The increase in nuclear localization of p21CIP11 observed after UV exposure is diminished in cells expressing exogenous CC3. Expression of CC3 also delays repair of oxidative DNA damage and leads to a decrease in levels of nucleoredoxin. Silencing of CC3 in CC3-positive cells has a modest delaying effect on repair of the UV induced damage, but has a much more significant negative affect on the translesion DNA synthesis after UV exposure. This could be related to the higher expression levels and increased nuclear localization of p21CIP11 in cells where expression of CC3 is silenced. Apparently, manipulation of the cellular levels of CC3 adversely affects DNA damage responses by affecting the levels and subcellular localization of relevant proteins.
Overall design 4 groups - U373 Neo Control, U373 CC3, U373 Neo Control UV, U373 CC3 UV. Each group has 3 biological replicates. Each biological replicates has 3 technical replicates.
Contributor(s) Fong S, Shtivelman E
Citation(s) 20374651
Submission date Mar 04, 2010
Last update date Aug 28, 2014
Contact name Sylvia Fong
Phone 510-420-4167
Organization name Bionovo Inc
Department Biology
Street address 5858 Horton St Suite 400
City Emeryville
State/province CA
ZIP/Postal code 94608
Country USA
Platforms (1)
GPL6254 Phalanx Human OneArray
Samples (36)
GSM518115 U373 CC3 Untreated Biological replicate 1, technical replicate 1
GSM518116 U373 CC3 Untreated Biological replicate 1, technical replicate 2
GSM518117 U373 CC3 Untreated Biological replicate 1, technical replicate 3
BioProject PRJNA124849

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20633_RAW.tar 93.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap