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| Status |
Public on Mar 10, 2010 |
| Title |
Differential Expression upon infection with Salmonella typhimurium (t0) |
| Organism |
Dictyostelium discoideum |
| Experiment type |
Expression profiling by array
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| Summary |
Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.
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| Overall design |
AX2 cells were grown in a 1000-fold excess of E.Coli B/r in Soerensen phosphate buffer under shaking. Cell were washed and resuspended at a concentration of 2 x 10^6 per ml with a 1000-fold excess of either S. typhimurium or E.Coli B/r in Soerensen phosphate buffer. After 0, 1, 2 and 3 hours cells were washed free of bacteria and total RNA was extracted for DNA microarray analysis.
For each timepoint three samples were taken and the experiment was done twice so that we had six samples for each timepoint (e.g. S1, S2, S3 and S1', S2', S3' ).
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| Citation(s) |
21824247 |
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| Submission date |
Mar 04, 2010 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Ludwig Eichinger |
| E-mail(s) |
ludwig.eichinger@uni-koeln.de
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| Phone |
+49 221 478 6928
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| Organization name |
Institute for Biochemistry 1
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| Lab |
AG Eichinger
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| Street address |
Joseph-Stelzmann-Strasse 52
|
| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL1972 |
Dictyostelium discoideum cDNA Microarray 6000 |
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| Samples (8)
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| GSM517135 |
SAE 13513874 GEO E1 t0 - cy3 vs S3 t0 - cy5 |
| GSM518023 |
SAE 13513874 GEO E1 t0 - cy5 vs S3 t0 - cy3 |
| GSM518024 |
SAE 13727433 GEO E3 t0 - cy3 vs S1 t0 - cy5 |
| GSM518042 |
SAE 13727460 GEO E3 t0 - cy5 vs S1 t0 - cy3 |
| GSM518087 |
SAE 13727629 GEO E3' t0 - cy3 vs S3' t0 - cy5 |
| GSM518103 |
SAE 13727630 GEO E3' t0 - cy5 vs S3' t0 - cy3 |
| GSM518104 |
SAE 13731063 GEO E1' t0 - cy3 vs S2' t0 - cy5 |
| GSM518105 |
SAE 13731064 GEO E1' t0 - cy5 vs S2' t0 - cy3 |
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| This SubSeries is part of SuperSeries: |
| GSE20688 |
Salmonella infected Dictyostelium discoideum |
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| Relations |
| BioProject |
PRJNA129601 |
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