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| Status |
Public on Jun 17, 2022 |
| Title |
Gene expression in human keratinocytes from psoriatic skin (directly into the lesion or in area without lesion) or healthy person (control) used for human tissue-engineered skin substitutes and co-cultured with T-lymphocyte |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of this study was to find deregulated genes between healthy and psoriatic T cell-enriched tissue-engineered models to develop a new therapeutic pathway in psoriasis treatment.
In this study, we used a tissue-engineered, two-layers (dermis and epidermis) human skin substitute enriched in T cells as a biomaterial to study both the cellular and molecular mechanisms involved in psoriasis’ pathogenesis.
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| Overall design |
3 human healthy keratinocytes population (at passage 3) from 3 healthy donors (18-, 46- and 49-years old), 4 human psoriatic keratinocytes population (at passage 3) from directly into the lesion of 4 psoriatic patients (36-, 46-, 49- and 64- years old) and 3 human psoriatic keratinocytes population (at passage 3) from area without lesion of three psoriatic patients (46-, 49- and 64- years old) co-cultured or not with T-lymphocyte from human tissue-engineered skin substitutes are analyzed by gene profiling.
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| Contributor(s) |
Rioux G, Turgeon F, Le-Bel G, Grenier C, Guérin S, Pouliot R |
| Citation(s) |
36139479 |
| Submission date |
Jun 16, 2022 |
| Last update date |
Oct 04, 2022 |
| Contact name |
Gaëtan Le-Bel |
| E-mail(s) |
gaetan.lebel17@gmail.com
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| Organization name |
CUO-Recherche
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| Lab |
Sylvain Guérin
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| Street address |
Local H2-00, Hôpital du Saint-Sacrement,1050 Chemin Sainte-Foy
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| City |
Québec |
| State/province |
Quebec |
| ZIP/Postal code |
G1S 4L8 |
| Country |
Canada |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (24)
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| GSM6250369 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, 001, rep1 |
| GSM6250370 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, T-lymphocyte, 001, rep1 |
| GSM6250371 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, 002, rep1 |
| GSM6250372 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, T-lymphocyte, 002, rep1 |
| GSM6250373 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, T-lymphocyte, 002, rep2 |
| GSM6250374 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, 002, rep1 |
| GSM6250375 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, T-lymphocyte, 002, rep1 |
| GSM6250376 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, T-lymphocyte, 002, rep2 |
| GSM6250377 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, 005, rep1 |
| GSM6250378 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, T-lymphocyte, 005, rep1 |
| GSM6250379 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, 006, rep1 |
| GSM6250380 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, T-lymphocyte, 006, rep1 |
| GSM6250381 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, T-lymphocyte, 006, rep2 |
| GSM6250382 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, 007, rep1 |
| GSM6250383 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, T-lymphocyte, 007, rep1 |
| GSM6250384 |
Cell Bank cultured skin substitute, human keratinocytes, healthy, T-lymphocyte, 007, rep2 |
| GSM6250385 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, 004, rep1 |
| GSM6250386 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, T-lymphocyte, 004, rep1 |
| GSM6250387 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, 004, rep1 |
| GSM6250388 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, T-lymphocyte, 004, rep1 |
| GSM6250389 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, 003, rep1 |
| GSM6250390 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, lesional, T-lymphocyte, 003, rep1 |
| GSM6250391 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, 003, rep1 |
| GSM6250392 |
Cell Bank cultured skin substitute, human keratinocytes, psoriatic, non-lesional, T-lymphocyte, 003, rep1 |
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| Relations |
| BioProject |
PRJNA850000 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206311_Processed_data.txt.gz |
2.7 Mb |
(ftp)(http) |
TXT |
| GSE206311_RAW.tar |
301.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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