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Series GSE206067 Query DataSets for GSE206067
Status Public on Jan 01, 2024
Title Themis directs T cell exhaustion by promoting PD-1 signaling and key transcription factors TCF-1 and TOX expression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We adopted scRNA-seq to examine whether Themis is required for Tex initiation. To strengthen the rigorousness of scRNA-seq analysis, we contransferred P14 WT and P14 Themis cKO cells, enabling them to experience the same viral burden and inflammatory environment. Then cells from all conditions (including naïve control cells) were combined and subjected to analysis.
 
Overall design T cells were FACS-sorted from lungs, or spleen of recipient mice which were co-transferred Themisflox/flox-dLck-Cre P14 and WT P14 Naive T cells 8 days after infection with Lymphocytic choriomeningitis virus Clone 13 strain, and subjected to single-cell RNA-seq by MARS-Seq protocol.
 
Contributor(s) Tang J, Jia X, Bao Y, Fu G
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 14, 2022
Last update date Jan 01, 2024
Contact name ChangChun Xiao
E-mail(s) cxiao@scripps.edu
Organization name The Scripps Research Institute
Department Department of Immunology and Microbiology
Street address North Torrey Pines Road
City La Jolla
ZIP/Postal code CA 92037
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (48)
GSM6241246 KO, Lung, 1- 1
GSM6241247 KO, Lung, 1- 2
GSM6241248 KO, Lung, 1- 3
Relations
BioProject PRJNA849008

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE206067_RAW.tar 31.2 Mb (http)(custom) TAR (of TXT)
GSE206067_cell_metadata.csv.gz 46.8 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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