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GEO help: Mouse over screen elements for information. |
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Status |
Public on Jan 01, 2024 |
Title |
Themis directs T cell exhaustion by promoting PD-1 signaling and key transcription factors TCF-1 and TOX expression |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We adopted scRNA-seq to examine whether Themis is required for Tex initiation. To strengthen the rigorousness of scRNA-seq analysis, we contransferred P14 WT and P14 Themis cKO cells, enabling them to experience the same viral burden and inflammatory environment. Then cells from all conditions (including naïve control cells) were combined and subjected to analysis.
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Overall design |
T cells were FACS-sorted from lungs, or spleen of recipient mice which were co-transferred Themisflox/flox-dLck-Cre P14 and WT P14 Naive T cells 8 days after infection with Lymphocytic choriomeningitis virus Clone 13 strain, and subjected to single-cell RNA-seq by MARS-Seq protocol.
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Contributor(s) |
Tang J, Jia X, Bao Y, Fu G |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Jun 14, 2022 |
Last update date |
Jan 01, 2024 |
Contact name |
ChangChun Xiao |
E-mail(s) |
cxiao@scripps.edu
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Organization name |
The Scripps Research Institute
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Department |
Department of Immunology and Microbiology
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Street address |
North Torrey Pines Road
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City |
La Jolla |
ZIP/Postal code |
CA 92037 |
Country |
USA |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (48)
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GSM6241249 |
KO, Spleen, 2- 1 |
GSM6241250 |
KO, Spleen, 2- 2 |
GSM6241251 |
KO, Spleen, 2- 3 |
GSM6241252 |
KO, Spleen, 1- 1 |
GSM6241253 |
KO, Spleen, 1- 2 |
GSM6241254 |
KO, Spleen, 1- 3 |
GSM6241255 |
KO, Spleen, 3- 1 |
GSM6241256 |
KO, Spleen, 3- 2 |
GSM6241257 |
KO, Spleen, 3- 3 |
GSM6241258 |
WT, Lung, 2- 1 |
GSM6241259 |
WT, Lung, 2- 2 |
GSM6241260 |
WT, Lung, 2- 3 |
GSM6241261 |
WT, Spleen, 3- 1 |
GSM6241262 |
WT, Spleen, 3- 2 |
GSM6241263 |
WT, Spleen, 3- 3 |
GSM6241264 |
WT, Spleen, 2- 1 |
GSM6241265 |
WT, Spleen, 2- 2 |
GSM6241266 |
WT, Spleen, 2- 3 |
GSM6241267 |
WT, Spleen, 1- 1 |
GSM6241268 |
WT, Spleen, 1- 2 |
GSM6241269 |
WT, Spleen, 1- 3 |
GSM6241270 |
WT, Lung, 1- 1 |
GSM6241271 |
WT, Lung, 1- 2 |
GSM6241272 |
WT, Lung, 1- 3 |
GSM6241273 |
WT, Lung, 3- 1 |
GSM6241274 |
WT, Lung, 3- 2 |
GSM6241275 |
WT, Lung, 3- 3 |
GSM6241276 |
KO, Lung, 2- 1 |
GSM6241277 |
KO, Lung, 2- 2 |
GSM6241278 |
KO, Lung, 2- 3 |
GSM6241279 |
KO, Lung, 3- 1 |
GSM6241280 |
KO, Lung, 3- 2 |
GSM6241281 |
KO, Lung, 3- 3 |
GSM6241282 |
WT, Naïve T, 1- 1 |
GSM6241283 |
WT, Naïve T, 1- 2 |
GSM6241284 |
WT, Naïve T, 1- 3 |
GSM6241285 |
WT, Naïve T, 1- 4 |
GSM6241286 |
WT, Naïve T, 1- 5 |
GSM6241287 |
WT, Naïve T, 1- 6 |
GSM6241288 |
KO, Naïve T, 1- 1 |
GSM6241289 |
KO, Naïve T, 1- 2 |
GSM6241290 |
KO, Naïve T, 1- 3 |
GSM6241291 |
KO, Naïve T, 1- 4 |
GSM6241292 |
KO, Naïve T, 1- 5 |
GSM6241293 |
KO, Naïve T, 1- 6 |
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Relations |
BioProject |
PRJNA849008 |
Supplementary file |
Size |
Download |
File type/resource |
GSE206067_RAW.tar |
31.2 Mb |
(http)(custom) |
TAR (of TXT) |
GSE206067_cell_metadata.csv.gz |
46.8 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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