|
| Status |
Public on May 09, 2023 |
| Title |
A network of DZF proteins controls alternative splicing regulation and fidelity. |
| Organisms |
Homo sapiens; synthetic construct |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
This SuperSeries is composed of the SubSeries listed below.
GSE206058: Paired-end total RNA-seq of triplicate biological replicate treatments of HEK-293 cells with negative control siRNAs or siRNAs targeting ZFR, ILF2, or ILF3.
GSE206059: eCLIP analysis of FLAG-ZFR binding in HEK-293 Flp-In TREx cell lines, with size-matched input control.
GSE206060: RNA Bind-n-Seq of recombinantly expressed and purified ZFR-ILF2 complexes, with PTBP1 as a positive control for successful library generation.
|
| |
|
| Overall design |
Please refer to individual series for information about experimental design and data processing.
|
| |
|
| Citation(s) |
37144502 |
| |
| Submission date |
Jun 14, 2022 |
| Last update date |
May 11, 2023 |
| Contact name |
J. Robert Hogg |
| E-mail(s) |
j.hogg@nih.gov
|
| Organization name |
National Heart, Lung, and Blood Institute
|
| Department |
Biochemistry and Biophysics Center
|
| Street address |
50 South Drive, Room 2341
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
| GPL27484 |
Illumina HiSeq 3000 (synthetic construct) |
|
| Samples (28)
|
|
| This SuperSeries is composed of the following SubSeries: |
| GSE206058 |
A network of DZF proteins controls alternative splicing regulation and fidelity [RNA-seq] |
| GSE206059 |
A network of DZF proteins controls alternative splicing regulation and fidelity [eCLIP-seq] |
| GSE206060 |
A network of DZF proteins controls alternative splicing regulation and fidelity [RNA Bind-n-Seq] |
|
| Relations |
| BioProject |
PRJNA848988 |
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