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Series GSE205707 Query DataSets for GSE205707
Status Public on Feb 23, 2023
Title Spatiotemporal mapping of immune and stem cell dysregulation after volumetric muscle loss
Organisms Canis lupus familiaris; Mus musculus
Experiment type Other
Summary Volumetric muscle loss (VML) is an acute trauma that results in persistent inflammation, supplantation of muscle tissue with fibrotic scarring, and decreased muscle function. The cell types, nature of cellular communication, and tissue locations that drive the aberrant VML response have remained elusive. Herein, we used spatial transcriptomics on mouse and canine models of VML and observed VML engenders a unique spatial pro-fibrotic pattern driven by crosstalk between fibrotic and inflammatory macrophages and mesenchymal derived cells. The dysregulated response was conserved between murine and canine VML models, albeit with varying kinetics, and impinged on muscle stem cell mediated repair. Targeting this circuit in a murine model resulted in increased regeneration and reductions in inflammation and fibrosis. Collectively, these results enhance our understanding of the cellular crosstalk that drives aberrant regeneration and provides further insight into possible avenues for fibrotic therapy exploration.
Overall design Spatial gene expression profiling was performed on flash-frozen, OCT embedded muscle tissue from mouse and canine post VML injury. Canine tissues were collected from two animals in different wound locations for a total of three spatial samples. Four replicates were generated for each treatment in mice (vehicle and ITD1) at 7 days post injury, and two replicates were generated for 14 days post injury.
Web link
Contributor(s) Larouche JA, Wallace EC, Spence BD, Johnson SA, Kulkarni M, Buras E, Brown BN, Badylak SF, Aguilar CA
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Submission date Jun 08, 2022
Last update date Feb 24, 2023
Contact name Carlos Andres Aguilar
Phone 734-764-8557
Organization name University of Michigan
Department Biomedical Engineering
Lab 2100 Gerstacker Bldg
Street address 2200 Bonisteel Blvd
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL25760 Illumina NovaSeq 6000 (Canis lupus familiaris)
Samples (18)
GSM6219587 599L
GSM6219588 600R
GSM6219589 1197L
BioProject PRJNA847234

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE205707_Canis_ST_list.RDS.gz 737.0 Mb (ftp)(http) RDS
GSE205707_Integrated_Merged_spGEX_SeuratObj_v3.RDS.gz 2.0 Gb (ftp)(http) RDS
GSE205707_Mus_ST_list_label_transfer.RDS.gz 363.4 Mb (ftp)(http) RDS
GSE205707_Mus_ST_list_label_transfer_1201.RDS.gz 441.6 Mb (ftp)(http) RDS
GSE205707_RAW.tar 20.3 Gb (http)(custom) TAR (of JSON, TIFF)
GSE205707_canis_merged_414T_415T_425T_426T.RDS.gz 342.5 Mb (ftp)(http) RDS
GSE205707_canis_merged_D0_D7_D14.RDS.gz 698.5 Mb (ftp)(http) RDS
GSE205707_merged_D7_D14.RDS.gz 234.6 Mb (ftp)(http) RDS
GSE205707_merged_filtered_599L_599R_600L_600R_1197L_1203L_1203R_1202L.RDS.gz 911.5 Mb (ftp)(http) RDS
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Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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