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Series GSE205679 Query DataSets for GSE205679
Status Public on Aug 05, 2022
Title The effect of 5-aminolevulinic acid on canine peripheral blood mononuclear cells
Organism Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary 5-aminolevulinic acid (ALA) is one of the natural amino acids and a product of the first heme synthesis pathway in mitochondria. Recently, a supplement containing ALA is available in Japan and used for the purpose of the enhancement of ATP synthesis via mitochondrial activity. In humans, the immunomodulatory effect of ALA has attracted attention as a new function of ALA, and its application to cancer, inflammatory disease, and autoimmune diseases are being investigated. In the present study, to evaluate the effects of ALA on canine immunity, we performed in vitro studies using peripheral blood mononuclear cells (PBMC) from healthy dogs. Heme oxygenase-1 protein was expressed in Madin-Darby Canine Kidney cells and PBMCs treated with 5-ALA and ferrous sodium citrate (SFC), which showed that ALA works in dogs as well as humans. When PBMCs were stimulated with concanavalin A (ConA), the addition of ALA resulted in a significant increase in interferon-gamma (IFN-γ) produced by ConA-stimulated PBMCs. A comprehensive genetic alteration using next-generation RNA sequences (RNA-seq) was performed. From the result of RNA-seq, ALA enhanced the gene expression of T cell immunity including Th1, Th2, and Th17 subsets. In particular, the IL-17 signaling pathway was significantly upregulated. Then, we confirmed that ALA promoted the production of interleukin (IL)-17A in ConA-stimulated PBMCs. Together, these findings reveal that ALA promotes heme synthesis in mitochondria and enhances ConA-induced T cell immune responses in canine PBMCs.
Overall design RNAseq analysis of PBMCs treated with or without ConA and ALA.
Contributor(s) Igase M, Iwatani N, Sakai A, Watanabe K, Mizukami Y, Mizuno T
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Submission date Jun 08, 2022
Last update date Aug 07, 2022
Contact name Yoichi Mizukami
Organization name Yamaguchi university
Department Science Research Center
Lab Institute of Gene Research
Street address Minamikogushi1-1-1
City Ube
State/province Yamaguchi
ZIP/Postal code 7558505
Country Japan
Platforms (1)
GPL21400 Illumina NextSeq 500 (Canis lupus familiaris)
Samples (12)
GSM6217219 PBMCs_1 without ALA and ConA
GSM6217220 PBMCs_1 with ALA
GSM6217221 PBMCs_1 with ConA
BioProject PRJNA847171

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Supplementary file Size Download File type/resource
GSE205679_RAW.tar 18.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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