GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE204981 Query DataSets for GSE204981
Status Public on Feb 06, 2023
Title Recycling of H2A-H2B provides short-term memory of chromatin states [ChOR-Seq]
Organisms Homo sapiens; Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The H3-H4 modification landscape is restored by parental histone recycling and post-replication modification of new histone H3-H4. How DNA replication impact on histone H2A-H2B is unknown. Here, we track H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub, H2BK120ub, and H2A.Z are recycled quantitatively and accurately during DNA replication. H2A-H2B are recycled symmetrically to daughter strands largely independent of known H3-H4 recycling pathways. Post-replication, H2A-H2B modifications are rapidly restored, and the rapid wave of H2AK119ub supports accurate restoration of H3K27me3. This work reveals epigenetic transmission of H2A-H2B modification during DNA replication and identifies H3-H4 and H2A-H2B crosstalk in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates reestablishment of slow, long-term chromatin state memory.
Overall design ChIP-Seq and ChOR-seq measuring chromatin occupancy for H3K27me3, H2AK119ub, H2A.Z, H2BK120ub, pan-H2A, pan-H3 in wildtype (WT) or Ring1B-mAID BAP1dTAG Ring1AKO mouse embryonic stem cells with corresponding clickedInputs controls in two biological replicates. CHOR-seq involves a 10min EdU labelling step, followed by a chase in medium without EdU (T15-T720; in min) or immediate harvesting (T0). Optional treatments are indicated. ClickedInputs are the corresponding inputs from the ChOR-seq time course where the EdU-labelled fraction has been clicked to Biotin, Streptavidin-purified and libraries generated.
Contributor(s) Flury V, Reverón-Gómez N, Alcaraz N, Stewart-Morgan K, Wenger A, Klose RJ, Groth A
Citation(s) 36750094
Submission date May 27, 2022
Last update date May 08, 2023
Contact name Anja Groth
Organization name Novo Nordisk Foundation Center for Protein Research
Street address Blegdamsvej 3B
City Copenhagen
ZIP/Postal code 2200
Country Denmark
Platforms (3)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL30172 NextSeq 2000 (Mus musculus)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (156)
GSM6203494 ChOR_T0_H2AK119ub_r1
GSM6203495 ChOR_T0_H2AK119ub_r2
GSM6203496 ChOR_T15_H2AK119ub_r1
This SubSeries is part of SuperSeries:
GSE204988 Recycling of H2A-H2B provides short-term memory of chromatin states
BioProject PRJNA843045

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 255.8 Mb (ftp)(http) BW 252.9 Mb (ftp)(http) BW 295.6 Mb (ftp)(http) BW 283.4 Mb (ftp)(http) BW 342.4 Mb (ftp)(http) BW 336.0 Mb (ftp)(http) BW 279.9 Mb (ftp)(http) BW 270.2 Mb (ftp)(http) BW 283.3 Mb (ftp)(http) BW 266.1 Mb (ftp)(http) BW 281.4 Mb (ftp)(http) BW 340.1 Mb (ftp)(http) BW 286.2 Mb (ftp)(http) BW 363.8 Mb (ftp)(http) BW 374.5 Mb (ftp)(http) BW 327.5 Mb (ftp)(http) BW 329.4 Mb (ftp)(http) BW 340.6 Mb (ftp)(http) BW 309.4 Mb (ftp)(http) BW 361.3 Mb (ftp)(http) BW 364.2 Mb (ftp)(http) BW 319.1 Mb (ftp)(http) BW 322.0 Mb (ftp)(http) BW 351.1 Mb (ftp)(http) BW 349.4 Mb (ftp)(http) BW 319.3 Mb (ftp)(http) BW 362.2 Mb (ftp)(http) BW 382.0 Mb (ftp)(http) BW 318.6 Mb (ftp)(http) BW 319.8 Mb (ftp)(http) BW 356.9 Mb (ftp)(http) BW 368.2 Mb (ftp)(http) BW 324.5 Mb (ftp)(http) BW 337.0 Mb (ftp)(http) BW 317.8 Mb (ftp)(http) BW 322.1 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap