GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE204932 Query DataSets for GSE204932
Status Public on Jan 25, 2023
Title A combinatorial approach to uncover a novel Integrator subunit [ChIP-seq I]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The Integrator complex is one of the most elusive transcriptional regulators in metazoans, deemed to be recruited after initiation to help establish and modulate paused RNAPII. Recent structural, biochemical, and functional evidence suggest that Integrator is composed of 14 subunits, assembling, and operating in a modular fashion. We employed proteomics and machine-learning (alphafold2) approaches to discover a novel Integrator subunit, INTS15. We report that INTS15 assembles primarily with the INTS10-INTS13-INTS14 module and interfaces with the Int-PP2A phosphatase module. Functional genomics analysis further reveals a role for INTS15 in modulating RNAPII pausing at a subset of genes in human cell lines.
Overall design C7orf26 (INTS15) was ChIPed in WT HeLa and HL-60 cells after stimulation. ChIP-seq for RNA polymerase II was done in HeLa cells that were treated with shRNAs for C7orf26 or Luciferase (control).
Contributor(s) Offley SR, Gardini A
Citation(s) 36920904
Submission date May 26, 2022
Last update date Sep 11, 2023
Contact name Sarah R Offley
Organization name The Wistar Institute
Lab Alessandro Gardini
Street address 3601 Spruce St
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19103
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (16)
GSM6202497 HL-60, NT, C7orf26, biol rep1
GSM6202498 HL-60, PMA, C7orf26, biol rep1
GSM6202499 HL-60, NT, C7orf26, biol rep2
This SubSeries is part of SuperSeries:
GSE204936 A combinatorial approach to uncover a novel Integrator subunit
BioProject PRJNA842740

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE204932_RAW.tar 2.1 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap