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Series GSE204741 Query DataSets for GSE204741
Status Public on Aug 03, 2022
Title Transcriptional regulation of the thymus master regulator Foxn1 [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary FOXN1 is a transcription factor critical for the development of both thymic epithelial cell (TEC) and hair follicle cell (HFC) compartments. However, mechanisms controlling its expression remain poorly understood. To address this question, we performed thorough analyses of the conservation and chromatin status of the Foxn1 locus in different tissues and states, and identified several putative cis-regulatory regions unique to TEC vs. HFC. Furthermore, experiments using genetically modified mice with specific deletions in the Foxn1 locus and additional bioinformatic analyses helped us identify key regions and transcription factors involved in either positive or negative regulation of Foxn1 in both TEC and HFC. Specifically, we identified SIX1 and FOXN1 itself, as key factors inducing Foxn1 expression in embryonic and neonatal TECs. Together, our data provide important mechanistic insights into the transcriptional regulation of the Foxn1 gene in TEC vs. HFC and highlight the role of FOXN1 in its autoregulation
Overall design ChIPseq profiles of Six1 and Sox9 binding in sorted thymic epithelial cells from embryonic or adult mice, respectively.
Contributor(s) Abramson J, Kadouri N
Citation(s) 36026441
Submission date May 24, 2022
Last update date Nov 03, 2022
Contact name Jakub Abramson
Organization name Weizmann Institute of Science
Department Department of Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM6190445 Six1_ChIP1
GSM6190446 Six1_WCE1
GSM6190447 SOX9_ChIP1
This SubSeries is part of SuperSeries:
GSE192875 Transcriptional regulation of the thymus master regulator Foxn1
BioProject PRJNA842065

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE204741_RAW.tar 2.7 Mb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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