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Series GSE204740 Query DataSets for GSE204740
Status Public on May 29, 2022
Title Global Stable-isotope Tracing Metabolomics Reveals System-wide Metabolic Alternations in Aging Drosophila
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Yet, delineating system-wide metabolic homeostasis at the whole-organism level remains non-trivial. Here, we develop a global isotope tracing metabolomics technology to measure labeled metabolites with a metabolome-wide coverage. Using Drosophila as an aging model organism, we probe the in vivo tracing kinetics with quantitative information on labeling patterns, extents and rates on a metabolome-wide scale. We curate a system-wide metabolic network to characterize metabolic homeostasis and disclose a system-wide loss of metabolic coordinations that impacts both intra- and inter-tissue metabolic homeostasis significantly during Drosophila aging. Importantly, we reveal an unappreciated metabolic diversion from glycolysis to serine metabolism and purine metabolism as Drosophila aging. The developed technology facilitates a system-level understanding of metabolic regulation in living organisms.
Overall design mRNA-seq of total RNAs from 3d and 30d flies, in triplicates, using Illumina Miseq.
Contributor(s) Wang R, Li J, Liu N, Zhu Z
Citation(s) 35725845
Submission date May 24, 2022
Last update date Jun 30, 2022
Contact name Ruohong Wang
Phone 15021841935
Organization name Chinese Academy of Sciences
Department IRCBC
Street address 100 Haike Road, 13th building
City Shanghai
State/province Not in US
ZIP/Postal code 200032
Country China
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (6)
GSM6190439 3D-1_S1_L001_R1_001
GSM6190440 3D-2_S2_L001_R1_001
GSM6190441 3D-3_S3_L001_R1_001
BioProject PRJNA842066

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Supplementary file Size Download File type/resource
GSE204740_Counts_Head.csv.gz 148.8 Kb (ftp)(http) CSV
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Processed data are available on Series record

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