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| Status |
Public on Mar 11, 2005 |
| Title |
carbon utilization in E. coli |
| Platform organism |
Escherichia coli K-12 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
The study investigated the effect of different carbon sources on E. coli global gene expression. We grew MG1655 cells aerobically in MOPS minimal medium with either glucose, glycerol, succinate, L-alanine, acetate, or L-proline as the carbon supply. Samples were taken from each culture at mid log phase and were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChipÒ E. coli Antisense Genome Arrays as recommended in the technical manual (www.affymetrix.com).
Keywords: parallel sample
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| Contributor(s) |
Liu M, Durfee T, Cabrera J, Zhao K, Jin D, Blattner F |
| Citation(s) |
15705577 |
| |
| Submission date |
Dec 03, 2004 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Mingzhu Liu |
| E-mail(s) |
mingzhuliu@wisc.edu
|
| Phone |
(608)-262-2534
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| Organization name |
University of Wisconsin-Madison
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| Street address |
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL199 |
[Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA91865 |
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