|
| Status |
Public on Jun 27, 2022 |
| Title |
Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [ESC RRBS] |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Parents transmit genetic and epigenetic information to their offspring. Maternal effect genes regulate the offspring epigenome to ensure normal development. Here we report that the epigenetic regulator SMCHD1 has a maternal effect on Hox gene expression and skeletal patterning. Maternal SMCHD1, present in the oocyte and preimplantation embryo, prevents precocious activation of Hox genes post-implantation. Without maternal SMCHD1, highly penetrant posterior homeotic transformations occur in the embryo. Hox genes are decorated with Polycomb marks H2AK119ub and H3K27me3 from the oocyte throughout early embryonic development; however, loss of maternal SMCHD1 does not deplete these marks. Therefore, we propose maternal SMCHD1 acts downstream of Polycomb marks to establish a chromatin state necessary for persistent epigenetic silencing and appropriate Hox gene expression later in the developing embryo. This is a striking role for maternal SMCHD1 in long-lived epigenetic effects impacting offspring phenotype.
|
| |
|
| Overall design |
Our overall design it to compare between Smchd1 control and Smchd1 maternally deleted samples. We have performed reduced representation bisulfite sequqencing (RRBS) in a differentiation timecourse in 2 replicate mESC of each genotype at 2 timepoints comparing the Smchd1 maternally deleted to the control, at the matched timepoint.
|
| |
|
| Contributor(s) |
Benetti N, Blewitt ME |
| Citation(s) |
35879318 |
| |
| Submission date |
May 23, 2022 |
| Last update date |
Aug 06, 2022 |
| Contact name |
Natalia Benetti |
| E-mail(s) |
nataliabenetti17@gmail.com
|
| Organization name |
WEHI
|
| Street address |
1G Royal Parade
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE183740 |
Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo |
|
| Relations |
| BioProject |
PRJNA841596 |
Less...
More...