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Series GSE203589 Query DataSets for GSE203589
Status Public on May 26, 2022
Title Transcriptomic profile of canine mammary ductal carcinoma
Organism Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary Mammary neoplasms are the most frequent in non-neutered bitches. Here we used next generation sequencing (NGS), to determine the transcriptional profile of samples of canine mammary ductal carcinomas and to contribute to the clarification of the importance of deregulated molecules in the molecular pathways involved in the disease. For this, samples of tumor tissue from mammary ductal carcinoma (without metastases) and non-tumor breast tissue from radical mastectomy of six bitches of different breeds and ages were used.
Overall design Samples of tumor tissue from mammary ductal carcinoma and adjacent non-tumor mammary tissue, from the radical mastectomy of 6 bitches of different breeds, not neutered, aged between 5 and 14 years, attended by the Small Animal Surgery Service of the Veterinary Hospital.RNA was extracted and was pooled with three samples each, forming four groups (two tumoral and two control samples). Each pool was used to build the four sequencing libraries. The cDNA libraries were built with the TruSeqTM Stranded Total RNA Sample Preparation Kit v2 (Illumina) and sequencing took place on the NextSeq 500 System platform (Illumina, USA) using the NextSeq 500/550 High Output v2 150 cycles (Illumina) Sequencing Kit, according to the manufacturer's instructions.
Contributor(s) Geysson Javier F, Adriana CF
Citation(s) 36982287
Submission date May 23, 2022
Last update date Jun 06, 2024
Contact name Geysson Javier Fernandez Garcia
Organization name UNESP
Street address Departamento morfologia IBB UNESPRubiao Junior
City Botucatu
State/province Sao Paulo
ZIP/Postal code 18603191
Country Brazil
Platforms (1)
GPL21400 Illumina NextSeq 500 (Canis lupus familiaris)
Samples (4)
GSM6176564 Adjacent Non-Tumor mammary tissue Pool 1
GSM6176565 Adjacent Non-Tumor mammary tissue Pool 2
GSM6176566 Mammary ductal Carcinoma Pool 1
BioProject PRJNA841500

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Supplementary file Size Download File type/resource
GSE203589_RAW.tar 390.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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