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Series GSE203321 Query DataSets for GSE203321
Status Public on Oct 31, 2023
Title BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state [scRNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Chromatin remodeling caused by SWI/SNF (BAF) complexes is essential for regulating cell fates. Of these, BRD9, an essential component of the non-canonical BAF (ncBAF) complex, is post-transcriptionally decayed in multiple types of cancer, especially in myelodysplastic syndrome (MDS). However, how BRD9 loss or ncBAF disruption alters the HSCs' integrity remains unknown. Here, we identify that Brd9 loss enhances chromatin accessibility, promoting myeloid-lineage skewing at the expense of B cell development in vivo, followed by MDS development. Brd9 significantly colocalizes with Ctcf, whose peaks are stoichiometrically enhanced by Brd9 loss, leading to increased chromatin loops with the larger chromatin domains intact. These Ctcf-associated chromatin loops newly occur in myeloid-related genes. These data uncover the unrecognized roles of BRD9/ncBAF in the cell fate specification of HSCs via fine-tuning chromatin loops and shed light on the mechanism of ncBAF-disrupted cancer.
 
Overall design Four weeks after birth, the primary mice received 20 mg/kg pIpC injection every other day for a total of 3 doses. Eight weeks after pIpC injection, the primary mice were sacrificed for sample preparation.
 
Contributor(s) Nomura M, Xiao M, Inoue D, Abdel-Wahab O
Citation(s) 38102116
Submission date May 18, 2022
Last update date Jan 02, 2024
Contact name Masaki Nomura
E-mail(s) masaki.nomura.fbri@gmail.com
Organization name Foundation for Biomedical Research and Innovation at Kobe
Street address 6-3-7 Minatojima Minamimachi, Chuo Ward
City Kobe
State/province Hyogo
ZIP/Postal code 650-0047
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM6166460 WT_HSPC
GSM6166461 Brd9_KO_HSPC
This SubSeries is part of SuperSeries:
GSE203322 BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state
Relations
BioProject PRJNA839419

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Supplementary file Size Download File type/resource
GSE203321_RAW.tar 240.0 Mb (http)(custom) TAR (of MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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