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Series GSE20330 Query DataSets for GSE20330
Status Public on Feb 15, 2010
Title Hairpin RNA induces secondary small interfering RNA synthesis and silencing in trans in fission yeast
Organism Schizosaccharomyces pombe
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary We provide evidence of the generation of Rdp1-mediated secondary siRNAs in vivo in fission yeast. Secondly, we show that the presence of Ago1-associated siRNAs does not guarantee robust silencing.
Overall design We obtained sequences from gel purified small RNAs of wt and rdp1delta fission yeast cells and FLAG-ago1 associated small RNAs.
Contributor(s) Simmer F, Buscaino A, Kos-Braun IC, Kagansky A, Boukaba A, Urano T, Kerr AR, Allshire RC
Citation(s) 20062003
Submission date Feb 15, 2010
Last update date May 15, 2019
Contact name Alastair Robert Kerr
Organization name University of Edinburgh
Department Wellcome Centre for Cell Biology
Lab Bioinformatics Core Facility
Street address 2.21 Michael Swann Building, Kings Buildings, Mayfield Road
City Edinburgh
State/province Midlothian
ZIP/Postal code EH9 3JR
Country United Kingdom
Platforms (2)
GPL9453 Illumina Genome Analyzer II (Schizosaccharomyces pombe)
GPL9854 Illumina Genome Analyzer (Schizosaccharomyces pombe)
Samples (3)
GSM509287 FLAG-ago1_siRNA
GSM509288 rdp1delta_siRNA
GSM509289 wt_siRNA
SRA SRP001896
BioProject PRJNA125397

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20330_RAW.tar 32.0 Mb (http)(custom) TAR (of BEDGRAPH, FNA)
GSE20330_constructs.fna.gz 2.0 Kb (ftp)(http) FNA
GSE20330_constructs.gff.gz 152 b (ftp)(http) GFF
SRA Run SelectorHelp
Processed data included within Sample table
Processed data provided as supplementary file
Raw data are available in SRA

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