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Series GSE203132 Query DataSets for GSE203132
Status Public on Jun 22, 2023
Title Transcriptomic diversity and overlapping clonality across subsets of antibody-secreting and memory B cells from spontaneous germinal centers
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Affinity matured self-reactive antibodies are found in autoimmune diseases like systemic lupus erythematous. Here we used fate-mapping reporter mice and single cell transcriptomics coupled to antibody repertoire analysis to characterize the post-germinal center (GC) B cell compartment in a new mouse model of autoimmunity. Antibody secreting cells (ASCs) and memory B cells (MemBs) from spontaneous GCs grouped into multiple subclusters. ASCs matured into two terminal clusters, with distinct secretion, antibody repertoire and metabolic profiles. MemBs contained FCRL5+ and CD23+ subsets, with different in vivo localization in the spleen. GC-derived FCRL5+ MemBs share transcriptomic and repertoire properties with atypical B cells found in aging and infection and localize to the marginal zone, suggesting a similar contribution to rapid recall responses. While transcriptomically diverse, ASC and MemB subsets maintained an underlying clonal redundancy. Therefore, self-reactive clones could escape subset-targeting therapy by perpetuation of self-reactivity in distinct subsets.
Overall design Chimeras were produced by transferring bone marrow from 564Igi and AicdaCreERT2-EYFP reporters (autoimmune) and AicdaCreERT2-EYFP and WT (immunized) into irradiate WT hosts. The latter were immunized with NP-OVA in alum, and boosted with NP-OVA. Fate-mapped AID-EYFP cells were isolated from spleens by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq.
Contributor(s) Castrillon C, Carroll M
Citation(s) 37341394
Submission date May 16, 2022
Last update date Jun 25, 2023
Contact name Carlos Castrillon
Organization name Harvard Medical School / Boston Childrens Hospital
Street address 200 Longwood Ave, Warren Alpert Building
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (16)
GSM6159405 M11 scRNAseq
GSM6159406 M13 scRNAseq
GSM6159408 M14 scRNAseq
BioProject PRJNA839019

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Supplementary file Size Download File type/resource
GSE203132_10X_clone-pass_germ-pass.tsv.gz 1.3 Mb (ftp)(http) TSV
GSE203132_RAW.tar 170.0 Mb (http)(custom) TAR (of FASTA, MTX, TSV)
GSE203132_sce.rds.gz 165.7 Mb (ftp)(http) RDS
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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