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Series GSE202915 Query DataSets for GSE202915
Status Public on Jan 12, 2023
Title CROP-seq + PIP-seq
Organism Homo sapiens
Experiment type Other
Summary We used PIP-seq with a CROP-seq library to evaluate the feability of PIP-seq for large pooled CRISPR screens
Overall design K562 CRISPRi cells were cultured in RPMI-1640 (Gibco #11875093) with 10% FBS (Thermo Fisher Scientific, #10438026) and 1% penicillin/streptomycin (Thermo Fisher Scientific, #15140148) in an incubator at 37°C with 5% CO2. K562 CRISPRi cells were transduced with a lentivirus library containing 138 sgRNA (Jost et al 2020) at a multiplicity of infection of 0.1. Lentivirus infected cells (BFP+) were sorted to high purity using a BD FACS Aria III (100 µMnozzle) and processed according to the PIP-seq scRNA-seq workflow.
Contributor(s) Clark I
Citation(s) 36879006
Submission date May 12, 2022
Last update date Apr 13, 2023
Contact name Iain Christopher Clark
Organization name University of California, Berkeley
Street address 374 Stanley Hall
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (1)
GSM6138430 CROP-seq RNA + sgRNA
This SubSeries is part of SuperSeries:
GSE202919 Microfluidics-free single-cell genomics with templated emulsification
BioProject PRJNA923463

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Supplementary file Size Download File type/resource 5.2 Mb (ftp)(http) ZIP
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