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| Status |
Public on Sep 13, 2024 |
| Title |
LINE-1 retrotransposons are reduced in bone of osteoporotic patients, correlate positively to BMD, and stimulate bone anabolism in transfected mesenchymal stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the role of LINE-1 in bone regeneration, we delivered synthetic L1 RNA to mesenchymal stem cells (MSC), from osteoporotic patients, induced to osteogenic differentiation.
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| Overall design |
First, mesenchymal stem cells (MSC) from femur of 4 osteoporotic patients (HUK9, HUK12, HUK16, HUK19) were isolated. MSC were then induced to osteogenic differentiation and transfected after 5 days with RFP mRNA (negative control) or L1 RNA. Samples were collected at day 6 (24h post-transfection) and day 10 (5 days post-transfection). Comparative gene expression profiling analysis of RNA-seq data for RFP mRNA and L1 RNA transfected cells was performed.
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| Contributor(s) |
Mangiavacchi A, Reppe S, Eggerschwiler B, Morelli G, Liu P, Saera-Vila A, Atinbayeva N, Casanova EA, Zhang H, Bensaddek D, Celii M, Della Valle F, Izpisua Belmonte JC, Cinelli P, Gautvik KM, Orlando V |
| Citation(s) |
38951609 |
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| Submission date |
Apr 28, 2022 |
| Last update date |
Sep 13, 2024 |
| Contact name |
Alfonso Saera-Vila |
| Organization name |
Sequientia Biotech
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| Street address |
Dr. Trueta 179
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| City |
Barcelona |
| ZIP/Postal code |
08005 |
| Country |
Spain |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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| GSM6071115 |
12L1, d6 |
| GSM6071116 |
16RFP, d6 |
| GSM6071117 |
16L1, d6 |
| GSM6071118 |
19RFP, d6 |
| GSM6071119 |
19L1, d6 |
| GSM6071120 |
9RFP, d10 |
| GSM6071121 |
9L1, d10 |
| GSM6071122 |
12RFP, d10 |
| GSM6071123 |
12L1, d10 |
| GSM6071124 |
16RFP, d10 |
| GSM6071125 |
16L1, d10 |
| GSM6071126 |
19RFP, d10 |
| GSM6071127 |
19L1, d10 |
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| Relations |
| BioProject |
PRJNA832979 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE201774_fpkm.txt.gz |
4.3 Mb |
(ftp)(http) |
TXT |
| GSE201774_newcounts.txt.gz |
5.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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