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Series GSE201424 Query DataSets for GSE201424
Status Public on May 12, 2022
Title An Engineered Multicellular Stem Cell Niche for the 3D Derivation of Human Myogenic Progenitors from iPSCs
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Fate decisions in the embryo are controlled by a plethora of microenvironmental interactions in a three-dimensional niche. To investigate whether aspects of this microenvironmental complexity can be engineered to direct myogenic human induced pluripotent stem cell (hiPSC) differentiation, we screened cell types present in the developmental or adult stem cell niche in heterotypic suspension embryoids. We identified embryonic endothelial cells and fibroblasts as highly permissive for myogenic specification of hiPSCs. After two weeks of sequential Wnt and FGF pathway induction, these three-component embryoids (iTCEs) are enriched in Pax7 positive embryonic-like myogenic progenitors (eMPs) that can be isolated by flow cytometry. Myogenic differentiation of hiPSCs in iTCEs relies on a specialized structural microenvironment and depends on MAPK, PI3K/AKT, and Notch signaling. After transplantation in a mouse model of Duchenne muscular dystrophy, eMPs repopulate the stem cell niche, reactivate after repeated injury and, compared to adult human myoblasts, display enhanced fusion and lead to stronger muscles. Altogether, we provide a two-week protocol for efficient and scalable suspension-based 3D derivation of Pax7 positive myogenic progenitors from hiPSCs.
Overall design QuantSeq from human induced pluripotent stem cell (hiPSC), three-component embryoids (TCEs) which comprise of murine growth arrested embryonic fibroblasts - GAeFib (Fibro), murine embryonic endothelial cells - eEC (Endo) and hiPSCs in comination and alone.
Contributor(s) Mashinchian O, De Franceschi F, Nassiri S, Michaud J, Migliavacca E, Aouad P, Metairon S, Pruvost S, Karaz S, Fabre P, Molina T, Stuelsatz P, Hegde N, Le Moal E, Dammone G, Dumont NA, Lutolf MP, Feige JN, Bentzinger CF
Citation(s) 35703167
Submission date Apr 25, 2022
Last update date Jul 06, 2022
Contact name Jerome Feige
Organization name Nestle Institute Of Health Sciences
Department Musculo-Skeletal Health
Street address EPFL Innovation Park, Building H
City Lausanne
ZIP/Postal code 1015
Country Switzerland
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL22245 Illumina HiSeq 2500 (Homo sapiens; Mus musculus)
Samples (24)
GSM6063482 Mono-aggregates_hiPSC_D7_pos_rep1
GSM6063483 Mono-aggregates_hiPSC_D7_pos_rep2
GSM6063484 Mono-aggregates_hiPSC_D7_pos_rep3
BioProject PRJNA831685

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE201424_htseq_summary2667_HsOnly_GEO.csv.gz 382.6 Kb (ftp)(http) CSV
GSE201424_htseq_summary2668_MmOnly_GEO.csv.gz 407.5 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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