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Series GSE200566 Query DataSets for GSE200566
Status Public on Nov 27, 2023
Title Prostaglandin E2 controls the metabolic adaptation of T cells to the intestinal microenvironment [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Immune cells must adapt to different environments during an immune response. We studied the adaptation of CD8+ T cells to the intestinal microenvironment and how this process shapes tissue resident CD8+ T cells (TRM) in the gut. CD8+ T cells progressively remodel their transcriptome and surface phenotype as they acquire gut residency, and downregulate mitochondrial gene expression. Human and mouse gut-resident CD8+ T cells have reduced mitochondrial mass, but maintain a viable energy balance to sustain function. We found that the intestinal microenvironment is rich in prostaglandin E2 (PGE2), which drives mitochondrial depolarization in CD8+ T cells. Consequently, they engage autophagy to clear depolarized mitochondria, and enhance glutathione synthesis to scavenge reactive oxygen species (ROS). Impairing PGE2 sensing promotes TRM accumulation, while tampering with autophagy and glutathione negatively impacts the TRM pool. Thus, a PGE2-autophagy-glutathione axis defines the metabolic adaptation of CD8+ T cells to the intestinal microenvironment, to ultimately influence the TRM pool.
 
Overall design We targeted 7 groups of CD8 T cells: mesenteric lymph node Naive (1), central memory (2), effector memory (3) and resident memory (4); Small instestine Lamina propria effector memory (5) and resident memory (6); Small intestine intraepithelial lymphocytes effector memory (7). 10^5 cells per target population from each of 3 biological replicates were isolated, processed and analyzed.
 
Contributor(s) Villa M, Sanin DE, Pearce EL
Citation(s) 38200005
Submission date Apr 11, 2022
Last update date Jan 18, 2024
Contact name Immunometabolism Department
E-mail(s) jcurti29@jhmi.edu
Organization name Johns Hopkins University
Department Immunometabolism
Street address 1650 Orleans Street
City Baltimore
State/province Maryland
ZIP/Postal code 21287
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (21)
GSM6037861 CD8_IEL_Resident_1
GSM6037862 CD8_IEL_Resident_2
GSM6037863 CD8_IEL_Resident_3
This SubSeries is part of SuperSeries:
GSE200568 Prostaglandin E2 controls the metabolic adaptation of T cells to the intestinal microenvironment.
Relations
BioProject PRJNA825380

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Supplementary file Size Download File type/resource
GSE200566_counts.tsv.gz 735.7 Kb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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