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Series GSE200554 Query DataSets for GSE200554
Status Public on May 01, 2024
Title Maternal Schistosomaisis Early bone marrow B cell scRNA CITEseq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We performed scRNA sequencing to find transcriptional differences in early B cell populations in the bone marrow of mice born to mothers chronically infected with Schistosoma mansoni. Cite-seq was used to help identify cluster types using surface protein markers.
Overall design Hematopoietic stem and progenitor cells and B cells were purified from bone marrow from the femur of mice born to S. mansoni infected and control mothers using a FACs Aria. Sorted cells were DAPI-CD3-CD4-CD8a-CD11b-CD11c-GR-1-Ter119-CD19-. CD19 was used in place of B220 to enrich for pre-pro B cells, but the antibody was ineffective, allowing for all B cell types to be sorted. After sorting, cells were stained with the cell surface proteins CD117(cKit) and Sca-1 with oligonucleotide-tagged antibodies to perform CITE-sequencing. Bone marrow from at least 3 mice were pooled before sorting. Cells were loaded into the 10X Genomics Chromium instrument according to the manufacturer's directions.
Contributor(s) Lisa G, Juan Marcos O, Keke F
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Submission date Apr 10, 2022
Last update date May 01, 2024
Contact name Keke Celeste Fairfax
Phone 810-581-5980
Organization name University of Utah
Department Pathology
Street address 15 N Medical Drive East
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (10)
GSM6037687 Pool_1_Uninfected_momminus_male_ADT
GSM6037688 Pool_1_Uninfected_momminus_male_GEX
GSM6037689 Pool_2_Uninfected_momminusfemale_ADT
BioProject PRJNA825262

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Supplementary file Size Download File type/resource
GSE200554_RAW.tar 291.2 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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