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Series GSE20052 Query DataSets for GSE20052
Status Public on Jan 23, 2011
Title Gene expression in the liver of ethanol-treated mice via gastric tube in multiple strains
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study tested a hypothesis that a population-based approach may identify genetic background-dependent and -independent genes/pathways that modulate alcohol-induced liver injury. A sub-chronic (4 weeks) intra-gastric alcohol infusion (up to 27 g/kg/day) model was used to control for alcohol intake in 15 genetically diverse inbred mouse strains. Urine, serum and liver markers of alcoholic steato-hepatitis were assessed and a wide range of liver damage was observed across the panel. Gene expression profiling was performed on liver tissue collected at the end of the study and statistical models, with strain, degree of liver injury, treatment, and their interaction terms as factors, were used to select transcripts associated with strain-dependent and -independent effects of alcohol on the liver. Translational control of hepatic protein synthesis, related to eukaryotic initiation factors (eiFs) and accessory proteins, was identified as genetic background-independent mode of alcohol-induced liver injury. Interestingly, immune response-related transcription factors signal transducer and activator of transcription 1 (Stat1) and nuclear factor, interleukin 3 regulated (Nfil3/E4bp4) are likely determinants of genetic background-dependent responses to liver injury and alcohol treatment, respectively. Our data demonstrate that a multistrain approach provides critical mechanistic information for understanding genetic factors influencing alcohol toxicity and facilitate identification of novel targets of therapeutic intervention.
Overall design A high-fat diet or EtOH-containing high-fat diet was administered to 15 strains of mice via gastric tube for 28 days. 3 replicates per strain/diet.
Inter-batch normalization was carried out using the ComBat procedure.

The supplementary file 'GSE20052_Readme.txt' contains a description of the replicates used for normalization. The 'GSE20052_US14702406_25148681*' files linked to the GSE20052 record are the raw data files for the replicates.
Contributor(s) Tsuchiya M, Gatti D, Kawaguchi A, Wright F, Kono H, Uehara T, Craig P, Kosik O, Shymoniak S, Melnyk S, Rusyn I
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Submission date Jan 26, 2010
Last update date Jan 12, 2017
Contact name Ivan Rusyn
Phone 919-843-2596
Fax 919-843-2596
Organization name University of North Carolina
Department Gillings School of Global Public Health
Lab Environmental Genomics Lab
Street address 0031 Michael Hooker Research Center
City Chapel Hill
State/province NC
ZIP/Postal code 27599-7431
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (90)
GSM501273 HFD_C3H/HeJ_6
GSM501274 HFD_C3H/HeJ_7
GSM501275 EtOH_C57BL/6J_19
BioProject PRJNA124319

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20052_RAW.tar 1.2 Gb (http)(custom) TAR (of TXT)
GSE20052_Readme.txt 3.5 Kb (ftp)(http) TXT
GSE20052_US14702406_251486811807_S01_GE2-v5_91_0806_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20052_US14702406_251486811811_S01_GE2-v5_91_0806_1_4.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812396_S01_GE2-v5_91_0806_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812407_S01_GE2-v5_91_0806_1_1.txt.gz 14.3 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812411_S01_GE2-v5_91_0806_1_4.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812412_S01_GE2-v5_91_0806_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812416_S01_GE2-v5_91_0806_1_4.txt.gz 14.1 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812422_S01_GE2-v5_91_0806_1_4.txt.gz 14.1 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812438_S01_GE2-v5_91_0806_1_1.txt.gz 14.2 Mb (ftp)(http) TXT
GSE20052_US14702406_251486812442_S01_GE2-v5_91_0806_1_4.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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