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| Status |
Public on Jun 01, 2022 |
| Title |
Tumor-associated Tregs obstruct anti-tumor immunity by promoting T cell dysfunction and restricting clonal diversity in tumor-infiltrating CD8+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated how accumulation of Tregs contributes to T cell dysfunction and clonal constriction of tumor-infiltrating CD8+ T cells. Resected melanoma atissues from tumor-bearing mice were analyzed. The clonal diversity of tumor-associated CD8+ T cells were evaluated by single-cell TCR sequencing respectively, at early or advanced tumor stages or under Treg depletion conditions. TCR-sequencing revealed a clonal shrinkage of tumor-infiltrating CD8+ T cells as tumor progressed, which was associated with reduced survival profile concomitant to increasing Treg proportions.
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| |
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| Overall design |
CD8+ T cells sorted from melanoma tissues in B16-F10 incoculated mice were processed for single-cell TCR sequencing
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| Contributor(s) |
Adeegbe D, Noyes D, Semidey-Hurtado J |
| Citation(s) |
35618289 |
| |
| Submission date |
Apr 08, 2022 |
| Last update date |
Mar 08, 2024 |
| Contact name |
Dennis Adeegbe |
| E-mail(s) |
Dennis.Adeegbe@moffitt.org
|
| Organization name |
Moffitt Cancer Center
|
| Department |
Immunology
|
| Street address |
13131 USF Magnolia Drive
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA824860 |
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