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| Status |
Public on Jun 29, 2022 |
| Title |
The Central Circadian Clock Protein CCA1 Regulates Seedling Growth and Spike Development in wheat (Triticum aestivum L.) |
| Organism |
Triticum aestivum |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The biological functions of circadian clock on growth and development have been well elucidated in model plants, while its regulatory roles in crop species, especially the roles on yield-related traits are poorly understood. Here, we characterize the core clock gene CCA1 homoeologs in wheat and studied their biological functions in seedling growth and spike development. TaCCA1 homoeologs exhibit typical diurnal expression patterns which are positively regulated by rhythmic histone modifications (H3K4me3, H3K9ac and H3k36me3). TaCCA1s are preferentially located in the nucleus and tend to form both homo- and heterodimers. TaCCA1 overexpression (TaCCA1-OE) transgenic wheat plants show disrupted circadian rhythmicity coupling with reduced chlorophyll and starch content, as well as biomass at seedling stage, also decreased spike length, grain number per spike and grain size at the ripening stage. Further studies using DNA affinity purification followed by deep sequencing (DAP-seq) indicates that TaCCA1 preferentially binds to sequences similar to “evening elements” (EE) motif in the wheat genome, particularly genes associated with photosynthesis, carbon utilization and auxin homeostasis, and decreased transcriptional levels of these target genes are observed in TaCCA1-OE transgenic wheat plants. Collectively, our study provides novel insights into a circadian-mediated mechanism of gene regulation to coordinate photo synthetic and metabolic activities in wheat, which is important for optimal plant growth and crop yield formation.
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| Overall design |
To explore the underlying regulation networks of TaCCA1s, the typical circadian rhythm-control transcription factor in wheat, we performed genome-wide TaCCA1-7D-binding profiles using DNA affinity purification followed by deep sequencing (DAP-seq).
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/35923880/
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| Contributor(s) |
Lu Z |
| Citation(s) |
35923880 |
| |
| Submission date |
Apr 07, 2022 |
| Last update date |
Feb 27, 2023 |
| Contact name |
Zefu Lu |
| E-mail(s) |
luzefu@live.com
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| Organization name |
Institute of Crop Sciences
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| Street address |
No.12 Zhongguancun South St.,Haidian District, Beijing, P.R.China
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| City |
Beijing |
| State/province |
Haidian |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL25409 |
Illumina NovaSeq 6000 (Triticum aestivum) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA824488 |
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