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Series GSE199982 Query DataSets for GSE199982
Status Public on Apr 05, 2022
Title Enamel organ mRNA expression in the limiited bedding and nesting (LBN) mouse model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In a systemic effort to survive environmental stress, organ systems fluctuate and adapt to overcome external pressures. The evolutionary drive back towards homeostasis, makes it difficult to determine if an organism experienced a toxic exposure to stress, especially in early prenatal and neonatal periods of development. Previous studies indicate that primary human teeth may provide historical records of experiences related to stressors during that early time window. To assess the molecular effects of early life adversity on enamel formation, we used a limited bedding and nesting (LBN) mouse model of early life adversity (ELA), to assess changes in enamel organ gene expression and enamel mineralization. Enamel of postnatal day 12 (P12) weight-matched ELA mice was more mineralized as compared control enamel. RNAseq showed 724 genes significantly upregulated in ELA enamel organs as compared to controls, and 574 significantly downregulated genes (DESeq2 p-value <= 0.05). Transcripts expressing the enamel matrix proteins amelogenin (Amelx) and enamelin (Enam) were among the top 4 most differentially expressed genes. The most significantly enriched GO and Reactome pathway was Extracellular Matrix Organization, while the most significantly enriched KEGG Pathways were Butanoate metabolism and Synthesis and Degradation of Ketone Bodies. qPCR analysis of weight-matched ELA and control enamel organs confirmed that expression of the ameloblast-specific proteins Amelx and Enam were highly upregulated in ELA mice. When evaluating molecular mechanisms for amelogenin upregulation, we found significantly increased expression of Dlx3, while transcripts for clock genes Per1 and Nrd1, which have also positively associated with amelogenin expression, were downregulated. These findings suggest that the developing enamel organs are sensitive to the pressures of early life adversity and produce structural biomarkers in tooth enamel mineral to reflect these changes. Together these results support the possibility that tooth enamel may contain biomarkers of cellular effects associated with systemic early life adversity.
Overall design Compare gene expression in enamel organs from an early life adversity mouse model as compared to controls
Contributor(s) Den Besten P
Citation(s) 37034482
Submission date Apr 01, 2022
Last update date May 02, 2023
Contact name Dawud abduweli uyghurturk
Organization name UCSF
Street address 513 Parnassus Ave.
City San Francisco
State/province California
ZIP/Postal code 94143
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM6001251 t-205-1-1
GSM6001252 t-205-1-3
GSM6001253 t-203-1-3
BioProject PRJNA822400

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Supplementary file Size Download File type/resource
GSE199982_gene_count.txt.gz 2.2 Mb (ftp)(http) TXT
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