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Series GSE199970 Query DataSets for GSE199970
Status Public on Apr 04, 2022
Title Sequencing and Quantitative Analysis of Skin Transcriptomes from Rats with EGFR Inhibitors Induced Rash
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Skin mRNA profiles of wild-type (WT) and EGFR Inhibitors (Gefitinib and Afatinib) induced rash rats were generated by deep sequencing, in triplicate, using Illumina GAIIx. Raw sequences were mapped to the rat reference sequence by Hisat2 v2.0.5. FeatureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene. Differential expression analysis was performed using the DESeq2 R package (1.16.1).
Overall design Skin mRNA profiles of wild-type (WT) and EGFR Inhibitors (Gefitinib and Afatinib) induced rash rats
Contributor(s) Zhang S, Liu X, Xie X, Chen L
Citation(s) 35618045
Submission date Apr 01, 2022
Last update date Jul 05, 2022
Contact name Shiyi Zhang
Organization name Shanghai Jiao Tong University
Street address 800 Dongchuan Rd
City Shanghai
ZIP/Postal code 200240
Country China
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (6)
GSM5997688 Skin WT rep1 [C412]
GSM5997689 Skin WT rep2 [C413]
GSM5997690 Skin Ge rep1 [G401]
BioProject PRJNA822384

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE199970_gene_count.txt.gz 1.3 Mb (ftp)(http) TXT
GSE199970_gene_fpkm.txt.gz 2.2 Mb (ftp)(http) TXT
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