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Series GSE19967 Query DataSets for GSE19967
Status Public on Jul 05, 2010
Title Gene Expression Profiling of siRNA Knock-Down of Six1, Six4 and Myogenin in C2C12
Organism Mus musculus
Experiment type Expression profiling by array
Summary Six1, Six4 and Myogenin are transcription factors that are known to be required for skeletal myogenesis. Currently, very little is known about the genes targeted by Six1 and Six4. Gene expression profiling when one or both transcription factors were knock-down by siRNA was performed to identify genes affected by their absence. We also hypothesized that Six1 and Six4 can work in cooperation with the myogenic regulatory factor (MRFs) family of transcription factors, such as Myogenin. Therefore, we performed the same type of experiment where the myogenin was knocked-down by siRNA to identify genes that are possibly regulated by the Six1 or Six4 in conjunction with Myogenin.
 
Overall design C2C12 Myoblasts were transfected with siRNA against Six1, Six4, Six1 with Six4, Myogenin, or control 24h before start of differentiation. The cells were allowed to differentiate in differentiation medium for 24h and were harvested for gene expression profiling. Four replicates per siRNA were performed.
 
Contributor(s) Chu A, Blais A
Citation(s) 20601407, 20956564
Submission date Jan 20, 2010
Last update date May 10, 2023
Contact name Alexandre Blais
E-mail(s) alexandre.blais@uottawa.ca
Organization name University of Ottawa
Street address 451 Smyth Road
City Ottawa
State/province ON
ZIP/Postal code K1H 8M5
Country Canada
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (20)
GSM498993 C2C12_siRNA_Control_R1
GSM498994 C2C12_siRNA_Control_R2
GSM498995 C2C12_siRNA_Control_R3
This SubSeries is part of SuperSeries:
GSE19988 Six1 and Six4 function in myoblast differentiation
Relations
BioProject PRJNA123701

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19967_RAW.tar 43.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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