|
| Status |
Public on Mar 01, 2024 |
| Title |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
Eukaryotic transcription factors (TFs) activate gene expression by recruiting cofactors to promoters. However, the relationships between TFs, promoters and their associated cofactors remain poorly understood. Here, we combine GAL4-transactivation assays with comparative CRISPR-Cas9 screens to identify the cofactors required by nine different TFs in human cells. Using this dataset, we associate key TFs with their cofactors, classify cofactors as ubiquitous or specific, discover novel transcriptional co-dependencies and demonstrate that certain TFs use the tail 2 and kinase submodules of Mediator to potentiate transcriptional elongation. By employing a reductionistic and comparative approach, we demonstrate that TFs do not display discrete mechanisms of activation. Instead, each TF is dependent on a unique combination of cofactors, which influences distinct steps in the transcriptional process. We also extend our screens to nine different core promoters to explore how core promoter elements influence cofactor dependence. Our data suggest that different classes of promoter are constrained by either initiation or pause release, which influences their dynamic range of gene expression and compatibility with specific cofactors. Overall, our comparative cofactor screens uncover the interplay between TFs, cofactors and core promoters and reveal general principles by which they influence transcription.
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| Overall design |
Refer to individual Series
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/38769457/
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| |
|
| Citation(s) |
38769457 |
| |
| Submission date |
Mar 18, 2022 |
| Last update date |
Jun 03, 2024 |
| Contact name |
Charles C Bell |
| E-mail(s) |
charles.bell@petermac.org
|
| Organization name |
Peter MacCallum Cancer Centre
|
| Department |
Cancer Research
|
| Street address |
VCCC, Gate 1, 14 Flemington Road
|
| City |
Melbourne |
| State/province |
VICTORIA |
| ZIP/Postal code |
3000 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (165)
|
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| This SuperSeries is composed of the following SubSeries:
|
| GSE198936 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [CRISPR TAD] |
| GSE198940 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [ChIP-seq] |
| GSE198942 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [RNA-seq] |
| GSE256168 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [CRISPR promoter] |
| GSE256170 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [ChIP-nexus] |
| GSE256173 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [SLAM-seq] |
| GSE256174 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [ZFP-TAD] |
| GSE256175 |
Comparative cofactor screens reveal the influence of transactivation domains and core promoters on the mechanisms of transcription [Degron ChIP] |
|
| Relations |
| BioProject |
PRJNA817542 |
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