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Series GSE19888 Query DataSets for GSE19888
Status Public on Mar 31, 2010
Title Expression data from HMC-1 mast cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We demonstrate that the G protein Gi3 is the cellular target of the adenosine A3 receptor (A3R). By using a cell permeable peptide comprising the C-terminal end of Gαi3 fused to an importation sequence (ALL1) as a selective inhibitor of Gi3 signaling, we show that by coupling to Gi3, the A3R stimulates multiple signaling pathways in human mast cells, leading to upregulation of cytokines, chemokines and growth factors.Following contact with activated T cell membranes, endogenous adenosine binds to and activates the A3R, resulting in Gi3-mediated signaling. Specifically, the majority of ERK1/2 signaling initiated by contact with activated T cell membranes, is mediated by Gi3, giving rise to ALL1-inhibitable cellular responses. These results unveil the physiological GPCR that couples to Gi3 and establish the important role played by this G-protein in inflammatory conditions that involve adenosine-activated mast cells.
We used microarrays to detail the effect of ALL1 on gene expression of HMC-1 cells activated directly by the A3 receptor, or by contact with activated T cell membranes.
Overall design The experiment consisted of 6 treatments in duplicates, a total of 12 samples. The cells were activated via two different activation pathways (A3 and T membranes), with or without pretreatment with the drug ALL1.
Contributor(s) Baram D, Sagi-Eisenberg R, Dekel O
Citation(s) 20190146
Submission date Jan 14, 2010
Last update date Dec 06, 2018
Contact name Dana Baram
Organization name Tel-Aviv University
Street address P.O. Box 39040
City Tel-Aviv
ZIP/Postal code 69978
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (12)
GSM497177 Untreated rep 1
GSM497178 Untreated rep 2
GSM497179 Incubated with ALL1 rep 1
BioProject PRJNA122029

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Supplementary file Size Download File type/resource
GSE19888_RAW.tar 21.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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