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Series GSE198484 Query DataSets for GSE198484
Status Public on Dec 04, 2022
Title RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The role of STAT3 signaling induced by stimulation of IL-6 cytokine family members such as OSM and CNTF on the angiogenic response of endothelial cells is still not fully understood. To assess the impact of this pathway on angiogenesis, this study compares human umbilical vein endothelial cells (HUVECs) with or without STAT3 knock-down introduced by siRNA and stimulation with VEGF, OSM+VEGF and CNTF+VEGF in the presence of the CNTF receptor (CNTFR).
Overall design mRNA profiles of HUVECs stimulated with VEGF (n=4), OSM+VEGF (n=4), or CNTF+CNTFR+VEGF (n=4) for 24h transfected with control siRNA. The samples were compared to HUVECs transfected with STAT3 siRNA and incubated by the same cytokines for 24 (n=4 each).
Contributor(s) Rapp J, Bucher F
Citation(s) 36458801
Submission date Mar 11, 2022
Last update date Dec 06, 2022
Contact name Julian Rapp
Organization name University Medical Center Freiburg
Street address Killianstra├če 5
City Freiburg im Breisgau
ZIP/Postal code 79106
Country Germany
Platforms (1)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (24)
GSM5949385 HUVEC, VEGF, Control siRNA, rep1
GSM5949386 HUVEC, VEGF, Control siRNA, rep2
GSM5949387 HUVEC, VEGF, Control siRNA, rep3
BioProject PRJNA815366

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE198484_RAW.tar 7.0 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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