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Status |
Public on Mar 14, 2022 |
Title |
CD8+ T cell responsiveness to anti-PD-1 is epigenetically regulated by Suv39h1 in melanomas [scRNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: We show that genetic ablation or pharmacological inhibition of H3K9-methyl transferase Suv39h1 delays tumor growth and potentiates tumor rejection by anti-PD-1.
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Overall design |
Suv39h1-KO and littermate WT mice were injected with B16-OVA tumor cells. On day 20, tumors were processed with Liberase and DNase I, stained with DAPI, CD45.2, TCRb, CD8, CD19, NK1.1, F4/80 and sorted on an Aria (BD) by gating on live, CD45.2+, (CD19, NK1.1, F4/80 negative cells) and CD8+ on PBS 0.04% BSA. Cells were loaded on a 10x Chromium instrument (10x Genomics) and libraries were prepared using a Single Cell 3′ Reagent Kit (V2 chemistry) (10x Genomics) according to manufacturer’s protocol. Sequencing reads were aligned to the mm10 transcriptome using the Cell Ranger suite with default parameters
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Contributor(s) |
Niborski L, Gueguen P |
Citation(s) |
35768432 |
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Submission date |
Mar 11, 2022 |
Last update date |
Jul 01, 2022 |
Contact name |
Paul Gueguen |
E-mail(s) |
paul.gueguen@unil.ch
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Organization name |
Institut Curie
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Department |
Inserm U932
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Street address |
26 rue D'Ulm
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City |
Paris |
ZIP/Postal code |
75005 |
Country |
France |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (9)
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This SubSeries is part of SuperSeries: |
GSE198423 |
CD8+ T cell responsiveness to anti-PD-1 is epigenetically regulated by Suv39h1 in melanomas |
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Relations |
BioProject |
PRJNA815054 |