|
| Status |
Public on Mar 01, 2024 |
| Title |
TGF-β specifies TFH versus TH17 cell fates in murine CD4+ T cells through c-Maf (Seq02, bulk-RNAseq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Naive murine CD4+ T cells from GREAT/SMART-17A mice were cultured under Th1 or Tfh(1 ng/ml TGF-β)-polarizing conditions in 96-well plates coated with anti-CD3/anti-CD28 for 3.5 days; sorted by flow cytometry on IFNg+ (Th1), or CXCR5-IL17A+ (Th17) and CXCR5+IL17A- (Tfh); and subjected to bulk RNA-seq.
|
| |
|
| Overall design |
Seq02: bulk RNA-seq: Th1IFNg+, Tfh(1)CXCR5+IL-17A–, Tfh(1)CXCR5+IL-17A–
|
| Web link |
https://www.science.org/doi/10.1126/sciimmunol.add4818
|
| |
|
| Contributor(s) |
Baumjohann D, Chang Y |
| Citation(s) |
38427718 |
| |
| Submission date |
Mar 10, 2022 |
| Last update date |
Mar 04, 2024 |
| Contact name |
Dirk Baumjohann |
| E-mail(s) |
dirk.baumjohann@uni-bonn.de
|
| Organization name |
University Hospital Bonn
|
| Department |
Medical Clinic III
|
| Lab |
Baumjohann Lab
|
| Street address |
Venusberg-Campus 1
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA814714 |
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