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Series GSE198207 Query DataSets for GSE198207
Status Public on May 11, 2023
Title Bulk TCRseq analysis on CD8+ T cells in FSCN1-KI bone marrow chimera mice following anti-CD4 and anti-PDL1 mAb treatment
Organism Mus musculus
Experiment type Other
Summary The repertoire of tumor-infiltrating T cells is a novel perspective to characterize effective anti-tumor T cell responses. Previous studies reported that the oligoclonal expansion in tumor T-cell repertoire is associated with anti-tumor effects. However, the contribution of the expansion of diverse T-cell clones remained unclear. We demonstrated that the polyclonal fraction of tumor-reactive T-cell repertoire consisting of relatively minor clones, increased in tumor-bearing mice treated with anti-PD-L1 or anti-CD4 monoclonal antibodies (mAbs), while the oligoclonal fraction consisting of major clones was unchanged. Moreover, the polyclonal fraction was enriched with progenitor exhausted T cells, essential for a durable anti-tumor response, and more dependent on CCR7+ migratory dendritic cells, responsible for priming tumor-reactive T cells in the tumor-draining lymph node (dLN). These results proposed that the expansion of diverse tumor-reactive clones, “clonal spreading,” is an important mechanism by which anti-PD-L1 and anti-CD4 treatments exert robust and durable anti-tumor T-cell responses.
 
Overall design CD8+ T cells from tumor, and CD8+ CD44high T cells from draining lymph node (dLN) were collected from B16F10 tumor-bearing Fscn1 knock-in BMC mice treated with anti-CD4 monoclonal antibody (mAb), anti-PD-L1 mAb or combination of anti-CD4 and anti-PD-L1 mAbs, with or without diphtheria toxin (DT) administration. Bulk TCR sequencing was performed, and T cell clones that overlap between tumor and dLN were identified.
 
Contributor(s) Aoki H, Tsunoda M, Ogiwara H, Shimizu H, Abe H, Abe T, Shichino S, Matsushima K, Ueha S
Citation(s) 36988477
Submission date Mar 09, 2022
Last update date May 12, 2023
Contact name Hiroyasu Aoki
E-mail(s) haoki-tky@rs.tus.ac.jp
Phone 08013746493
Organization name Tokyo University of Science
Department Research Institute for Biomedical Sciences
Lab Division of Molecular Regulation of Inflammatory and Immune Diseases
Street address 2669 Yamazaki
City Noda
State/province Chiba
ZIP/Postal code 278-0022
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (54)
GSM5940716 B16_Tumor_CD4PDL1_DT_CD8_1
GSM5940717 B16_Tumor_CD4PDL1_DT_CD8_2
GSM5940718 B16_Tumor_CD4PDL1_DT_CD8_3
This SubSeries is part of SuperSeries:
GSE198211 Clonal spreading of tumor-infiltrating T cells underlies the durable antitumor effects of PD-1 blockade and anti-CD4 monoclonal antibody
Relations
BioProject PRJNA814225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE198207_ICIrep_FscnTCRseq_GEO_0221_table1.xls.gz 7.5 Kb (ftp)(http) XLS
GSE198207_RAW.tar 15.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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