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| Status |
Public on Oct 18, 2023 |
| Title |
EZH2 inhibition with GSK343 reduces H3K27me3 deposition at various repetitive elements in splenic B cells [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
EZH2 is a histone methyltransferase that deposits the repressive histone H3 lysine 27 trimethylation (H3K27me3). This mark has been reported to silence expression of various repetitive elements in the genome. We sought to identify the effect of EZH2 inhibition on H3K27me3 peaks and their overlap with repetitive elements in splenic B cells. We generated a novel mutant mouse line whose ability to detect induced repeat element expression has been abrogated. We report that EZH2 inhibition decreases the number of H3K27me3 peaks in both WT and mutants compared to control.
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| Overall design |
Genome-wide examination of H3K27me3 deposition in splenic B cells upon EZH2 inhibition
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| |
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| Contributor(s) |
Dick FA, Kim SJ |
| Citation(s) |
37934086 |
| |
| Submission date |
Mar 08, 2022 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Frederick Andrew Dick |
| E-mail(s) |
fdick@uwo.ca
|
| Organization name |
Western University
|
| Department |
Pathology
|
| Lab |
A4-VRL LRCP
|
| Street address |
790 Commissioners Road E
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A 4L6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
|
| GSM7653208 |
WT B cells H3K27me3 input DMSO Rep2 |
| GSM7653209 |
WT B cells H3K27me3 ChIP GSK343 Rep1 |
| GSM7653210 |
WT B cells H3K27me3 input GSK343 Rep1 |
| GSM7653211 |
WT B cells H3K27me3 ChIP GSK343 Rep2 |
| GSM7653212 |
WT B cells H3K27me3 input GSK343 Rep2 |
| GSM7653213 |
Mutant B cells H3K27me3 ChIP DMSO Rep1 |
| GSM7653214 |
Mutant B cells H3K27me3 input DMSO Rep1 |
| GSM7653215 |
Mutant B cells H3K27me3 ChIP DMSO Rep2 |
| GSM7653216 |
Mutant B cells H3K27me3 input DMSO Rep2 |
| GSM7653217 |
Mutant B cells H3K27me3 ChIP GSK343 Rep1 |
| GSM7653218 |
Mutant B cells H3K27me3 input GSK343 Rep1 |
| GSM7653219 |
Mutant B cells H3K27me3 ChIP GSK343 Rep2 |
| GSM7653220 |
Mutant B cells H3K27me3 input GSK343 Rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE198232 |
Cytosolic pattern recognition receptors respond to EZH2-inhibition induced viral mimicry response that eliminates splenic B cells |
|
| Relations |
| BioProject |
PRJNA813876 |
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