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Series GSE197721 Query DataSets for GSE197721
Status Public on Sep 13, 2023
Title Genome-wide maps of hematopoietic stem and progenitor cells in mice bone marrow.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To investigate whether the skeletal retardation observed in NG2-cre/Pot1afl/fl mice affected hematopoiesis.
Methods: Whole bone marrow cells mRNA profiles of 2-week-old wild-type (NG2-cre/Pot1awt/wt) and Pot1a knockout (NG2-cre/Pot1afl/fl) mice were generated by deep sequencing, using Illumina NextSeq 500.
Results: We compared the relative sizes of these subpopulations between the NG2-cre/Pot1afl/fl and control mice and found a significant reduction in cells of the B-cell lineage in NG2-cre/Pot1afl/fl marrow. Cell subpopulations associated with B-cell differentiation from hematopoietic stem/progenitor cells (HSPCs) were identified according to the expression of established marker genes.
Conclusions: Bone marrow microenvironments composed of POT1a-deleted MSPCs impair B-cell development.
 
Overall design Single cell mRNA profiles of bone marrow HSCs and progenitor cells in NG2-cre/Pot1afl/fl and control mice.
 
Contributor(s) Nakashima K, Kunisaki Y, Semba Y, Nogami J, Arai F
Citation(s) 37773433
Submission date Mar 01, 2022
Last update date Oct 19, 2023
Contact name Yuichiro Semba
E-mail(s) semba.yuichiro.208@m.kyushu-u.ac.jp
Organization name Kyushu University
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (9)
GSM5928799 Pot1awt/wt BM cells rep1
GSM5928800 Pot1awt/wt BM cells rep2
GSM5928801 Pot1awt/wt BM cells rep3
Relations
BioProject PRJNA811630

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Supplementary file Size Download File type/resource
GSE197721_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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