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| Status |
Public on Feb 20, 2024 |
| Title |
Protozoan-Derived Cytokine-Transgenic Macrophages Reverse Hepatic Fibrosis. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophage therapy for liver fibrosis is on the cusp of meaningful clinical utility. Due to the heterogeneities of macrophages, it is urgent to develop safer macrophages with a more stable and defined phenotype for the treatment of liver fibrosis. Herein, a new macrophage-based immunotherapy using macrophages stably expressing a pivotal cytokine from Toxoplasma gondii, a parasite that infects ≈ 2 billion people is developed. It is found that Toxoplasma gondii macrophage migration inhibitory factor-transgenic macrophage (Mφtgmif) shows stable fibrinolysis and strong chemotactic capacity. Mφtgmif effectively ameliorates liver fibrosis and deactivates aHSCs by recruiting Ly6Chi macrophages via paracrine CCL2 and polarizing them into the restorative Ly6Clo macrophage through the secretion of CX3CL1. Remarkably, Mφtgmif exhibits even higher chemotactic potential, lower grade of inflammation, and better therapeutic effects than LPS/IFN-γ-treated macrophages, making macrophage-based immune therapy more efficient and safer. Mechanistically, TgMIF promotes CCL2 expression by activating the ERK/HMGB1/NF-κB pathway, and this event is associated with recruiting endogenous macrophages into the fibrosis liver. The findings do not merely identify viable immunotherapy for liver fibrosis but also suggest a therapeutic strategy based on the evolutionarily designed immunomodulator to treat human diseases by modifying the immune microenvironment.
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| Overall design |
mRNA profiles of mouse leukemia cell of monocyte macrophage RAW264.7 (Mφ), Mφ stably expressing LV-ZsGreen (LV-Mφ), Mφ stably expressing LV-TgMIF-ZsGreen (TgMIF-Mφ), LPS and IFN-γ treated Mφ(LPS/IFNγ-Mφ). Two or three independent experiments were performed for each condition.
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| Contributor(s) |
Chen Y, Wang J, Zhou N, Fang Q, Cai H, Du Z, An R, Liu D, Chen X, Wang X, Li F, Yan Q, Chen L, Du J |
| Citation(s) |
38247166 |
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| Submission date |
Mar 01, 2022 |
| Last update date |
May 22, 2024 |
| Contact name |
Ying Chen |
| E-mail(s) |
cy.juice@aliyun.com
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| Phone |
13966667603
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| Organization name |
Anhui Medical University
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| Street address |
No. 81 Meishan Road, Hefei
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| City |
Anhui |
| State/province |
China |
| ZIP/Postal code |
230032 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA811434 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197639_a-d_mergefpkm.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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